Hu-Cytokines

Recombinant Human G-CSF

Cat No. Size Price Add Cart
HHM-GC-0010 10ug $ 150.00 Add Cart
HHM-GC-0100 100ug $ 1005.00 Add Cart
HHM-GC-1000 1000ug $ 4285.00 Add Cart

Product Specifications

•      Expression of Human Proteins in Human Cells

•      Extreme low Endotoxin

•      High Purity

•      Animal Free and Xeno Free

•      Tag Free

 

Source: Human cells derived

Structure: Glycosylated monomer

Purity: >95% by SDS-PAGE

Endotoxin Level: <0.5EU/ug

Molecular Weight: 21-25kDa

Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line).

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

 

Protein Description

Granulocyte colony stimulating factor (G-CSF) is a hematopoietic growth factor belonging to the CSF family. G-CSF is mainly expressed in monocyte/ macrophage lineage. It is also expressed in cells of mesodermal origin, including vascular endothelial cells, fibroblasts, and mesothelial cells. G-CSF acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

 

References

Nagata S et al. (1986) Nature 319,415-418.

Shirafuji N et al. (1989) Exp Hematol. 17,116-119.