Hu-Cytokines

Recombinant Human HGF

Cat No. Size Price Add Cart
HST-HF-0010 10ug $ 225.00 Add Cart
HST-HF-0100 100ug $ 1005.00 Add Cart
HST-HF-1000 1000ug $ 4285.00 Add Cart

Product Specifications

•      Expression of Human Proteins in Human Cells

•      Extreme low Endotoxin

•      High Purity

•      Animal Free and Xeno Free

•      Tag Free

Source: Human cells derived

Structure: Glycosylated monomer

Purity: >95% by SDS-PAGE

Endotoxin Level: <0.5EU/ug

Molecular Weight: 70kDa

Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate the proliferation of the monkey epithelial cell line 4MBr-5.

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

 

Protein Description

HGF is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. HGF regulates many physiological processes including proliferation, scattering, morphogenesis and survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries.

 

References

Di Renzo MF, et al. (1991) Oncogene 6, 1997-2003.

Hammond DE, et al. (2004) Curr. Top. Microbiol. Immunol. 286,21.

Bottaro DP,et al. (1991) Science 251,802-804.