Recombinant Human IGF-1
|Cat No.||Size||Price||Add Cart|
|EME-IF1-0010||10ug||$ 35.00||Add Cart|
|EME-IF1-0100||100ug||$ 85.00||Add Cart|
|EME-IF1-1000||1000ug||$ 235.00||Add Cart|
Source: E. coli derived
Structure: Monomer, with an additional 13 amino acid residues at N terminal.
Purity: >95% by SDS-PAGE
Endotoxin Level: <1EU/ug
Molecular Weight: 9kDa
Formulation: Lyophilized from a 0.2μm filtered solution in 50mM NaAOC without carrier protein
The activity was measured by its ability to stimulate the proliferation of 3T3 mouse fibroblast cells.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 10mM acetic acid containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Insulinlike growth factor I(IGF-1), also known as somatomedin C, is a secreted protein which belongs to the insulin family. IGF-1 binds IGFI R, IGFII R, and the insulin receptor, although its effects are mediated primarily by IGFI R, which is structurally and functionally related to insulin but has a much higher growth-promoting activity.IGF-1 regulates proliferation and differentiation of a wide variety of cell types. The human mature IGF- 1 composes of 70 amino acids and three disulfide bonds with a MW of 7.6kD.The recombinant IGF- 1 is modified with additional 13 amino acids at N terminal to increase the activity and stability.
Ornitz DM, et al. (1996) J. Biol. Chem. 271, 15292- 15297.
Abraham JA, et al. (1986) Cold Spring Harb. Symp. Quant. Biol. 51, 657-668.