Non-HuCytokines

Recombinant Human IGF-1

Cat No. Size Price Add Cart
EME-IF1-0010 10ug $ 35.00 Add Cart
EME-IF1-0100 100ug $ 85.00 Add Cart
EME-IF1-1000 1000ug $ 235.00 Add Cart

Source: E. coli derived

Structure: Monomer, with an additional 13 amino acid residues at N terminal.

Purity: >95% by SDS-PAGE

Endotoxin Level: <1EU/ug

Molecular Weight: 9kDa

Formulation: Lyophilized from a 0.2μm filtered solution in 50mM NaAOC without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate the proliferation of 3T3 mouse fibroblast cells.

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 10mM acetic acid containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

 

Protein Description

Insulinlike growth factor I(IGF-1),  also  known as somatomedin C, is a secreted protein which belongs to the insulin family. IGF-1 binds IGFI R, IGFII R, and the insulin receptor, although its effects are mediated primarily by IGFI R, which is structurally and functionally related to insulin but has a much higher growth-promoting activity.IGF-1 regulates proliferation and differentiation of a wide variety of cell types. The human mature IGF- 1 composes of 70 amino acids and three disulfide bonds with a MW of 7.6kD.The recombinant IGF- 1 is modified with additional 13 amino acids at N terminal to increase the activity and stability.

 

References

Ornitz DM, et al. (1996) J. Biol. Chem. 271, 15292- 15297.

Abraham JA, et al. (1986) Cold Spring Harb. Symp. Quant. Biol. 51, 657-668.