Hu-Cytokines

Recombinant Human TNFα

Cat No. Size Price Add Cart
HIM-TNa-0010 10ug $ 87.00 Add Cart
HIM-TNa-0100 100ug $ 625.00 Add Cart
HIM-TNa-1000 1000ug $ 4000.00 Add Cart

Product Specifications

•      Expression of Human Proteins in Human Cells

•      Extreme low Endotoxin

•      High Purity

•      Animal Free and Xeno Free

•      Tag Free

 

Source: Human cells derived

Structure: Glycosylated homotrimer

Purity: >95% by SDS-PAGE

Endotoxin Level: <0.5EU/ug

Molecular Weight: 17kDa

Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by the cytotoxicity in L-929 cell in the presence of actinomycin D.

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

 

Protein Description

Tumor necrosis factor alpha (TNF-α) is an EGF- related polypeptide growth factor that signals through the TNFRSF1A/TNFR1 and TNFRSF1B/ TNFBR. It  is  mainly  secreted by macrophages and also produced by monocytes, keratinocytes, and  various  tumor  cells.  TNF-α   stimulates the proliferation of a wide range  of  epidermal and epithelial cells. TNF-α is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of  cachexia.  Under certain  conditions it can stimulate cell proliferation and induce cell differentiation, impair regulatory T cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation.

 

References

Pennica D, et al. (1984) Nature 312, 724-729.

Nie H, et al. (2013) Nat. Med. 19, 322-328.

Friedmann E, et al. (2006) Nat. Cell Biol. 8,843- 848.