Hu-Cytokines

Recombinant Human NGF-β

Cat No. Size Price Add Cart
HST-N-0010 10ug $ 105.00 Add Cart
HST-N-0100 100ug $ 306.00 Add Cart
HST-N-1000 1000ug $ 1650.00 Add Cart

Product Specifications

•      Expression of Human Proteins in Human Cells

•      Extreme low Endotoxin

•      High Purity

•      Animal Free and Xeno Free

•      Tag Free

 

Source: Human cells derived

Structure: Glycosylated monomer

Purity: >95% by SDS-PAGE

Endotoxin Level: <0.5EU/ug

Molecular Weight: 13kDa

Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

 

Protein Description

Nerve growth factors (NGFs ) is composed of three noncovalently linked subunits, α, β, and γ, while the β subunit, called NGF-β exhibits all the biological activities ascribed to NGF. Recombinant human NGF-β is a homodimer of two 120 amino acid polypeptides. NGF-β is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF-β is a well characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF-β can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF-β has also been shown to have biological effects on non- neuronal tissues.

 

References

Wijeyewickrema LC, et al. (2010) J. Biol. Chem. 285, 11793-11799.

Cirulli F, et al. (2009) Front. Neuroendo. 30, 379-395.