Hu-Cytokines

Recombinant Human M-CSF

Cat No. Size Price Add Cart
HHM-MC-0010 10ug $ 195.00 Add Cart
HHM-MC-0100 100ug $ 825.00 Add Cart
HHM-MC-1000 1000ug $ 4285.00 Add Cart

Product Specifications

•      Expression of Human Proteins in Human Cells

•      Extreme low Endotoxin

•      High Purity

•      Animal Free and Xeno Free

•      Tag Free

 

Source: Human cells derived
Structure: Glycosylated homodimer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 35-40kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate   the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line).

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

M-CSF (Macrophage Colony stimulating Factor) is a hematopoietic stem cell factor and produced by various cell types including lymphocytes, fibroblasts, endothelial cells, myoblasts, monocytes, and osteoblasts. It is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Recombinant human M-CSF is a 35-40 kDa homodimeric and glycosylated.

References

Kawasaki ES, et al (1985). Science 230 (4723): 291-6.
Stein J, Borzillo GV, Rettenmier CW (1990). Blood 76 (7): 1308-14.