Recombinant Human LIF
|Cat No.||Size||Price||Add Cart|
|EST-LIF-0010||10ug||$ 125.00||Add Cart|
|EST-LIF-0100||100ug||$ 595.00||Add Cart|
|EST-LIF-1000||1000ug||$ 2900.00||Add Cart|
Source: E. coli derived
Purity: >95% by SDS-PAGE
Endotoxin Level: <1EU/ug
Molecular Weight: 19.5 kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein
The specific activity was determined by the dose-dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
LIF is a pleiotropic member of the IL6 family of cytokines produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF functions through a heterodimeric receptor complex containing a ligand binding subunit, LIF R, and a signal transducing subunit gp130 with complex effects. LIF either induces or inhibits differentiation in different cell environments. LIF promotes long-term maintenance of pluripotency in embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of protein synthesis by hepatocytes, and differentiation of cholinergic nerves acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes.
Metcalfe, S.M. (2011) Genes Immun. 12:157.
Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
Yue X, et al. (2015) Cancer Cell Microenviron. 2015;2(3). pii: e877.