Hu-Cytokines

Recombinant Human IL-3

Cat No. Size Price Add Cart
HCT-I3-0010 10ug $ 205.00 Add Cart
HCT-I3-0100 100ug $ 785.00 Add Cart
HCT-I3-1000 1000ug $ 3245.00 Add Cart

Product Specifications

•      Expression of Human Proteins in Human Cells

•      Extreme low Endotoxin

•      High Purity

•      Animal Free and Xeno Free

•      Tag Free

 

Source: Human cells derived

Structure: Glycosylated monomer

Purity: >95% by SDS-PAGE

Endotoxin Level: <0.5EU/ug

Molecular Weight: 19kDa

Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

 

Reconstitution

Briefly  centrifuge  the  vial  before  opening.  It  is recommended to reconstitute the protein in sterile PBS  containing  at  least  0.1%  human  or bovine serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

 

Protein Description

Interleukin-3 (IL-3) is belongs to hematopoietic growth factors. It is a secreted protein. IL-3 can improve the body's natural response to disease as part of the immune system.  IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells (as opposed to lymphoid progenitor cells where differentiation is stimulated by IL-7) as well as stimulates proliferation of all cells in the myeloid lineage (erythrocytes, megakaryocytes, granulocytes, monocytes, and dendritic cells) in conjunction with other cytokines, such as EPO, GM-CSF, TPO and IL-6. Additionally, IL-3 aids in embryo implantation and placental development, was found to be decreased both in pregnant women with antiphospholipid syndrome and in animal models of APS. IL-3 also participates in the response of the organism to various types of stress.

References

Yang YC, et al. (1986). Cell 47(1): 3-10.
Martinez-Moczygemba M, et al (2003). J. Allergy Clin. Immunol. 112 (4): 653–65.