Recombinant Mouse WNT3A
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- Expression of Mouse Proteins in CHO Cells
- Extreme low Endotoxin
- High Purity
- Tag Free
Source: Mouse cells derived
Structure: Glycosylated monomer
Purity: >75% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 38-41kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein
Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a generate 100-fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS with 1% Chaps containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Mouse Wnt3a shows 96% amino acid identity to human Wnt3a protein.
This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
Lu, D and et al. (2011) Oncotarget. 2: 587 - 587
Taranjit S. Gujral, et al, (2014). Cell. 159: 844-856
Kahn.M (2014) Nature Reviews Drug Discovery 13: 513–532