Recombinant Human/Mouse WNT5A
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- Expression of Mouse Cells
- Extreme low Endotoxin
- High Purity
- Tag Free
Source: Human cells derived
Structure: Glycosylated monomer
Purity: >85% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: ~45kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein
The inhibitory activity of Wnt5a on the canonical Wnt pathway has been measured using TCF-based Wnt reporter stable cell line stimulated by mouse Wnt3a. IE50 of Wnt5a is about 2.5 ng/mL in the presence of 1 ng/mL of mouse Wnt5a.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS with 1% Chaps containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. Protein Wnt5a is a protein that is encoded by the WNT5A gene. This gene is a member of the WNT gene family. Mature mouse Wnt5a is 100% identical in amino acids to mature human Wnt5a after being secreted from expression cells. Wnt5a protein can inhibits or activates TCF-based Wnt signaling.
This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
Bauer M, et al. PLoS ONE 8(11): e80526.
Janda CY, et al. (2012) Science.337(6090): 59-64.
Milkels AJ, et al. (2006) PLoS Biol, 4: e115.