Hu-Cytokines

Recombinant Human IL-21

Cat No. Size Price Add Cart
HCT-I21-0010 10ug $ 195.00 Add Cart
HCT-I21-0100 100ug $ 1026.00 Add Cart
HCT-I21-1000 1000ug $ 5300.00 Add Cart

Product Specifications

Source: Human cells derived
Structure: Glycosylated monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.1EU/ug
Molecular Weight: ~15.4kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by a cell proliferation assay using human N1186 T cells. The ED50 for this effect is about 0.5-5ng/ml. The activity was measured by a cell proliferation assay using human N1186 T cells. The ED50 for this effect is about 0.5-5ng/ml.

Reconstitution 

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

IL-21 is a typical family I cytokine with broad pleiotropic actions and is primarily produced by T follicular helper cells,Th17, and natural killer T cells in response to antigenic stimulation. Il-21 generally enhances antigen-specific responses of immune cells. It controls the activation, proliferation, differentiation, cytotoxicity, and survival of various target immune cells.  Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant Human IL-21 is a 15.4 kDa protein consisting of 132 amino acid residues.

References

Parrish Novak, et al. (2000) Nature 408,57.
Spolski R, et al.(2008) Annu Rev Immunol 26,57.
Mehta DS, et al.(2004) Immune Rev 202,84.