Media/Reagents

Recombinant Human EGF

Cat No. Size Price Add Cart
EME-EF-0010 10ug $ 45.00 Add Cart
EME-EF-0100 100ug $ 75.00 Add Cart
EME-EF-1000 1000ug $ 245.00 Add Cart

Source: E. coli derived
Structure: Monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <1EU/ug
Molecular Weight: 6 kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by its ability to stimulate   the proliferation of 3T3 mouse fibroblast cells.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Epidermal growth factor (EGF) belogs to growth factors that stimulates cell growth, proliferation, and differentiation by binding to EGFR. The effects of EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion. Additionally, It stimulates the proliferation of various epidermal and epithelial cells.  

References

Harris RC, et al. (March 2003). Experimental Cell Research 284 (1): 2–13.
Venturi S, et al. (2009). Nutrition and Health 20 (2): 119–134.