Hematopoietic SCs

Recombinant Human TPO

Cat No. Size Price Add Cart
HHM-TP-0010 10ug $ 240.00 Add Cart
HHM-TP-0100 100ug $ 1680.00 Add Cart
HHM-TP-1000 1000ug $ 3750.00 Add Cart

Product Specifications

 

Source: Human cells derived
Structure: Glycosylated monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 80-85kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate the proliferation of human Mo7e cells.

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

 

Protein Description

Recombinant human thrombopoietin  expressed in engineered human cells. TPO belongs to the EPO/TPO protein family. TPO protein is an 80 to 85 kDa monomeric glycoprotein. TPO is primarily produced in the liver and regulates the formation of megakaryocytes and platelets. C terminal domain glycosylation is thought to be important for the secretion of TPO from cells and for survival of TPO in the circulation.

 

References

Kitamura, T, et al. (1989) J. Cell Physiol. 140, 323-334.
Kaushansky, K, (1998) NEJM 339, 746-754