Hematopoietic SCs

Recombinant Human FLT3 Ligand

Cat No. Size Price Add Cart
HHM-FT-0010 10ug $ 158.00 Add Cart
HHM-FT-0100 100ug $ 1075.00 Add Cart
HHM-FT-1000 1000ug $ 3270.00 Add Cart

Product Specifications

 

Source: Human cells derived
Structure: Glycosylated monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 24-30kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Human Flt3 Ligand is a 24-30kDa glycoprotein with a 158 amino acid.Flt3 Ligand is expressed by T cells, bone marrow and thymic fibroblasts. The predominant biologically active form is membrane- bound isoform, which can be proteolytically cleaved to generate a biologically active soluble isoform. Flt3 Ligand synergizes well with a number of other colony stimulating factors and interleukins to regulate proliferation of early hematopoietic cells by activating Flt3.

 

References

Lyman SD. et al.1995 Oncogene. 10 (1), 149-57.
Hannum C., et al.(1994) Nature 368,643-648.
Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.