Hematopoietic SCs

Recombinant Human IL-6

Cat No. Size Price Add Cart
HIM-I6-0010 10ug $ 104.00 Add Cart
HIM-I6-0100 100ug $ 785.00 Add Cart
HIM-I6-1000 1000ug $ 3245.00 Add Cart

Product Specifications

Source: Human cells derived
Structure: Glycosylated monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 26-30kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

 

Activity Assay

The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

 

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

 

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

 

Protein Description

Human interleukin 6 (IL-6) is a 26-30 kDa glycoprotein with 183 amino acids in length. IL-6 is a pleiotropic cytokine that plays an important role in the final differentiation of B cells into Ig secreting cells. IL-6 acts on B cells, T cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is expressed by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes.

 

References

Yasukawa, K., et al. (1987) EMBO J. 6, 2939-2945.
Garbers C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
Schwantner A. et al. (2004) J. Biol. Chem.279 (1), 571-576.