Recombinant Human IL-21
|Cat No.||Size||Price||Add Cart|
|HCT-I21-0010||10ug||$ 195.00||Add Cart|
|HCT-I21-0100||100ug||$ 1026.00||Add Cart|
|HCT-I21-1000||1000ug||$ 5300.00||Add Cart|
- Expression of Human Proteins in Human Cells
- Extreme low Endotoxin
- High Purity
- Animal Free and Xeno Free
- Tag Free
Source: Human cells derived
Structure: Glycosylated monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.1EU/ug
Molecular Weight: ~15.4kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein
The activity was measured by a cell proliferation assay using human N1186 T cells. The ED50 for this effect is about 0.5-5ng/ml. The activity was measured by a cell proliferation assay using human N1186 T cells. The ED50 for this effect is about 0.5-5ng/ml.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
IL-21 is a typical family I cytokine with broad pleiotropic actions and is primarily produced by T follicular helper cells,Th17, and natural killer T cells in response to antigenic stimulation. Il-21 generally enhances antigen-specific responses of immune cells. It controls the activation, proliferation, differentiation, cytotoxicity, and survival of various target immune cells. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant Human IL-21 is a 15.4 kDa protein consisting of 132 amino acid residues.