Product Application: Anti-CD3 & CD28 Antibody Nanoparticles
Primary Application:
- Activation and Expansion of Human T Cells: These nanoparticles are designed to stimulate the immune response of T cells, making them ideal for research and therapeutic applications involving T cell activation and proliferation.
Specific Uses:
- Immunotherapy: In the development of cancer immunotherapies, these nanoparticles can be used to enhance T cell-mediated tumor recognition and killing.
- Autoimmune Diseases: They can be used to study the mechanisms of autoimmune diseases and potentially develop therapeutic strategies to modulate T cell activity.
- Vaccines: These nanoparticles might be incorporated into vaccine formulations to improve immune responses and enhance vaccine efficacy.
- Basic Research: They are valuable tools for studying T cell biology, signaling pathways, and immune responses.
Key Features and Benefits:
- Dual Targeting: The presence of both anti-CD3 and anti-CD28 antibodies ensures strong T cell activation and proliferation.
- Ease of Use: The provided protocol is straightforward, making it accessible for researchers with varying levels of experience.
- Flexibility: The nanoparticles can be used for both short-term activation and long-term expansion of T cells.
- Compatibility: They are compatible with various downstream applications, including flow cytometry and functional assays.
In summary, Anti-CD3 & CD28 antibody nanoparticles offer a versatile and effective tool for researchers and scientists working in the fields of immunology, cancer research, and autoimmune diseases.
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Please Note: This Product's CoA, Data Sheet and SDS are not available.
Product Description
Anti-CD3 & CD28 antibody nanoparticles are ideal to be used to activate human T cells. The nanoparticles are conjugated with both of anti-human CD3 antibody and anti-human CD28 antibody. They act as antigen-presenting cells and activate resting T cells from peripheral blood mononuclear cells (PBMCs) as well as purified T cells.
Product Contents
Anti-CD3 & CD28 antibody nanoparticles are provided in phosphate buffered saline (PBS) with 0.01% sodium azide. 1 ml of suspension solution is enough for expansion of 4X107 human T cells. Store at 4°C upto 6 months.
Additional material required
Product Applications
Human T cell activation/expansion
Protocol
For optimal results from the nanoparticles, it is recommended that the nanoparticles are washed prior to addition to samples.
1. Vortex MagVig nanoparticles for 10-20 seconds.
2. Transfer the desired volume of nanoparticles to a tube. Add equal volume of Buffer, and vortex to mix.
3. Separate the nanoparticles from the solution by placing the magnet on the side of the tube for 2-5 min and remove the supernatant carefully (with magnet still on the side). Note: A clear precipitate containing dark brown colored nanoparticles should become visible on the side of the microcentrifuge tube.
4. Remove the tube from the magnet and resuspend the washed nanoparticles in the same volume of Culture Medium as the initial volume taken to wash.
Activation of Human T Cells
2. Incubate in a humidified CO2 incubator at 37°C, according to your specific requirements
3. Harvest the activated T cells and use directly for further analysis.
4. For flow cytometry applications, remove nanoparticles prior to staining. Place the tube on a magnet for 2-5 min to separate the nanoparticles from the solution. Transfer the supernatant containing the cells to a new tube.
Expansion of Human T Cells
1. Add 25 ul of SI-nanoparticles to 1 ml cells (1X106/ml).
2. Add 30 U/ml rIL-2.
3. Incubate in a humidified CO2 incubator at 37°C, according to your specific requirements.
4. Check cells daily. Add rIL-2 every other day. When the cell density exceeds 2.5 X 106 cells/ml or medium turns yellow, split cultures to a density of 0.5-1 X 106 cells/ml in culture medium containing 30U/ml rIL-2.
5. Count cells on day 5, 7, 9 and 11.
6. For human pan-T cells, recommend to culture cells for 11 days.
Table 1. Guild for the use of bead volumes.
|
Seeding T cell numbers
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8X104
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1X106
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50X106
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|
bead volume
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2 ul
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25 ul
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1.25 ml
|
|
rIL-2
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30 U/ml
|
30 U/ml
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30 U/ml
|
|
seeding medium volume
|
100-200 ul
|
1-2 ml
|
50-100 ml
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Anti-CD3 & CD28 antibody nanoparticles
- Brand: StemImmune LLC
- Product CAT#: HCT-Sbe-0001 (1ml), HCT-Sbe-0005 (5ml), HCT-Sbe-0020 (20ml)
- Availability: Out Of Stock
Available Options
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