Product Application: Human BMP-4

Primary Application:

Stem Cell Differentiation: BMP-4 is a crucial factor in inducing the differentiation of mesenchymal stem cells (MSCs) into bone and cartilage, making it invaluable for tissue engineering and regenerative medicine.
Bone and Cartilage Formation: It plays a vital role in bone and cartilage development, repair, and regeneration.
Tooth Development: BMP-4 is involved in tooth formation and growth.
Embryonic Development: It is essential for mesoderm induction and limb formation during embryonic development.
Fracture Repair: BMP-4 promotes bone healing and fracture repair.

Specific Uses:

Tissue Engineering: BMP-4 can be used to create bone and cartilage grafts for various medical applications.
Drug Discovery: It serves as a valuable tool for studying bone diseases and developing potential therapeutic targets.
Basic Research: BMP-4 is essential for understanding the mechanisms of bone and cartilage development and regeneration.

Key Features and Benefits:

High Purity and Low Endotoxin: Ensures optimal biological activity and minimal off-target effects.
Animal-Free and Xeno-Free: Suitable for sensitive applications and avoids potential contamination.
Tag-Free: Preserves the native structure and function of the protein.
High Activity: Demonstrated by its ability to induce alkaline phosphatase production in ATDC-5 cells.
Convenient Formulation: Lyophilized format allows for easy storage and reconstitution.

In summary, Human BMP-4 is a valuable tool for researchers and scientists working in the fields of stem cell biology, tissue engineering, regenerative medicine, and bone and cartilage research.

Citations for Recombinant Human BMP-4

Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.

Product Specifications

Expression of Human Proteins in Human Cells
Extreme low Endotoxin
High Purity
Animal Free and Xeno Free
Tag Free

Source: Human cells derived
Structure: Non-glycosylated homodimer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 34-40kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by its ability to induce alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line).

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Bone morphogenetic protein 4 (BMP-4) is a member of transforming growth factor β family that includes more than 20 structurally related bone growth factors. BMP-4 is widely expressed from early embryogenesis through dulthood. It plays an important role in cartilage and bone formation, mesoderm induction, tooth development, limb formation and fracture repair. Mature human BMP-4 is a 116 amino acids glycoprotein and normally found as a homodimer. Dimerization is facilitated by a disulfide bridge formed between the monomer, which contains three intrachain disulfide bridges arranged in a cystine knot motif. BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.