Product Specifications
- Expression of Human Proteins in Human Cells
- Extreme low Endotoxin
- High Purity
- Animal Free and Xeno Free
- Tag Free
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein
Activity Assay
IFNγ stimulates STAT1 phosphorylation in human lung microvascular endothelial cells. HLMVECs were serum starved in EBM-2 medium containing 2% FBS for overnight then treated with La IFNγ for 5 minutes at the indicated concentrations ranging from 0.5 to 500 ng/ml. Phosphorylated STAT1 (Tyr701) was detected by phosphor STAT1 antibody by Western Blot.
Reconstitution
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Protein Description
Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the type II class of interferons. IFNγ signaling in antigen-presenting cells, and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response. IFNγ is critical for innate and adaptive immunity against viral, protozoal, bacterial infections. IFNγ is secreted by Th1, cytotoxic T cells, NKT cells and NK cells. IFNγ is particularly suitable for human CIK activation along with IL-2.
References
Gray PW, et al (August 1982). Nature 298 (5877): 859-63.
Jiang J et al. (2013) J Transl Med 11:83.
Stem Cells Studies | |
Description | Immunotherapy DC-CIK Car-T and T-cells activation |
Documentations | |
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Recombinant Human IFNγ
- CAT#: HST-IFg-0010 (10ug), HST-IFg-0100 (100ug), HST-IFg-1000 (1000ug)
- Availability: In Stock
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