• Proteinase K (Ready-to-Use)

Product Application: Proteinase K Ready-to-Use

Primary Application:

  • Protein Digestion and Purification: Proteinase K is widely used for the complete digestion of proteins, which is essential for various applications, such as protein purification, mass spectrometry, and peptide analysis.

Specific Uses:

  • Protein Purification: It can be used to remove contaminants from protein samples, improving the purity of the target protein.
  • Western Blotting: Proteinase K can be used to digest unwanted proteins in lysates before Western blotting, reducing background noise and improving signal clarity.
  • Mass Spectrometry: It is used to prepare protein samples for mass spectrometry analysis by digesting them into smaller peptides.
  • Peptide Synthesis: Proteinase K can be used to cleave synthetic peptides at specific sites, generating fragments for further analysis or modification.
  • DNA Extraction: Proteinase K is commonly used to degrade proteins during DNA extraction, preventing protein contamination of the DNA sample.

Key Features and Benefits:

  • Broad Substrate Specificity: Digests a wide range of proteins, making it versatile for various applications.
  • Native State Digestion: Can digest proteins in their native, folded state, simplifying sample preparation.
  • Heat Stability: Remains active at elevated temperatures, allowing for efficient digestion under various conditions.
  • EDTA Stability: Can be used in buffers containing EDTA without affecting its activity.
  • Ready-to-Use Format: Convenient for immediate use, saving time and effort.

In summary, Proteinase K Ready-to-Use is a valuable tool for researchers and scientists working in a variety of fields, including biochemistry, molecular biology, and proteomics. Its broad substrate specificity, heat stability, and ease of use make it an essential enzyme for protein digestion and purification applications.

Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.

 

Source: Yeast derived
Purity: >98% by SDS-PAGE
Endotoxin Level: N/O
Molecular Weight: 28.93kDa

Activity Assay

Proteinase K activity can be measured using hemoglobin as the substrate

Description

Proteinase K is classified as a serine protease and falls under the Peptidase family S8. Serine proteases are enzymes that utilize a serine residue in their active site for proteolysis. Proteinase K exhibits broad substrate specificity, meaning it can cleave a wide range of peptide bonds in proteins. This characteristic makes it valuable for various applications, including protein purification and DNA extraction. One of the notable features of Proteinase K is its ability to degrade proteins even in their native, folded state. This is unlike many other proteases that require denaturation of proteins before cleaving peptide bonds. Proteinase K can be inhibited by serine protease inhibitors such as phenylmethylsulfonyl fluoride (PMSF). These inhibitors bind to the active site serine residue and prevent enzymatic activity. Unlike some other proteases, Proteinase K's activity is unaffected by sulfhydryl modifying reagents. These reagents typically target cysteine residues, but Proteinase K's catalytic activity is not dependent on such residues. EDTA (ethylenediaminetetraacetic acid) is a chelating agent commonly used to sequester metal ions. The statement suggests that Proteinase K remains stable when stored or used in a buffer containing EDTA. This stability is important to maintain the enzyme's activity.

Reconstitution

Stemimmune Ready-to-Use Proteinase K do not need reconstitution.

Stability & Storage

StemImmune Ready-to-Use Proteinase K can be stored in room temperature

Proteinase K Ready-to-Use Solution - 20 mg/ml

Instructions

1) Technical specifications

StemImmune Ready-to-Use Proteinase K
Format Ready-to-Use Solution
Amount & Activity 20mg/ml, >600mAU/ml

 

 

 

Proteinase K is widely used in various molecular biology and biochemistry applications due to its ability to effectively digest proteins without being hindered by the protein's native structure. It's commonly utilized in DNA and RNA extraction protocols, as well as in removing protein contaminants from nucleic acid samples.

2) StemImmune Ready-to-Use Proteinase K:

YPE-PK-001 (1ml) and YPE-PK-010 (10ml) Solution concentration (20mg/ml)

      3) Suggested Work Concentration: 0.05~1mg/ml

Note: Proteinase K interacts with nucleases. To ensure maximum Proteinase K activity, use water that has been treated with 0.1% DEPC if you dilute use

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Proteinase K (Ready-to-Use)

  • Product CAT#: YPE-PK-001 (ml), YPE-PK-010 (10ml)
  • Availability: In Stock

Available Options


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