Product Specifications

• Expression of Human Proteins in Human Cells
• Extreme low Endotoxin
• High Purity
• Animal Free and Xeno Free
• Tag Free

Source: Human cells derived
Structure: Non-glycosylated homodimer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 25kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by its ability to inhibit the IL-4 induced proliferation in mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Transforming growth factor beta 3(TGF β3) is one of three closely related mammalian members of the large TGF β superfamily, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. The mammalian TGF-β3 controls a vast array of biological processes including immune regulation, cell proliferation, epithelial-mesenchymal transition, and the bone formation. It is generally recognized to facilitate chondrogenic differentiation of precursor cells. It may also have a dose-dependent inhibitory effect on osteogenesis. Recombinant Human TGF-β3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

References