Product Specifications

Expression of Human Proteins in Human Cells
Extreme low Endotoxin
High Purity
Animal Free and Xeno Free
Tag Free

Source: Human cells derived

Structure: Glycosylated monomer

Purity: >95% by SDS-PAGE

Endotoxin Level: <0.5EU/ug

Molecular Weight: 24-30kDa

Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by its ability to stimulate the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

FLT3L purity and Activity Assay

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Human Flt3 Ligand is a 24-30kDa glycoprotein with a 158 amino acid. Flt3 Ligand is expressed by T cells, bone marrow and thymic fibroblasts. The predominant biologically active form is membrane- bound isoform, which can be proteolytically cleaved to generate a biologically active soluble isoform. Flt3 Ligand synergizes well with a number of other colonies stimulating factors and interleukins to regulate proliferation of early hematopoietic cells by activating Flt3.