Product Specifications

• Expression of Human Proteins in Human Cells
• Extreme low Endotoxin
• High Purity
• Animal Free and Xeno Free
• Tag Free

Source: Human cells derived
Structure: Glycosylated homodimer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 39-45kDa homotrimer
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by its ability to stimulate the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Vascular endothelial growth factor (VEGF) is a potent growth and angiogenic cytokine. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. Humans express alternately spliced isoforms, and VEGF-165 is the most abundant and potent isoform. VEGF stimulates angiogenesis, vasculogenesis and endothelial cell growth, induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Recombinant human VEGF-165 is a 39-45kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptides.