Product Application: Human iPS Cell Chemical Reprogramming Kit
Primary Application:
- Generation of Human Chemically Induced Pluripotent Stem Cells (hCiPSCs): This kit enables the efficient and scalable reprogramming of human somatic cells, such as fibroblasts and adipose-derived stromal cells, into hCiPSCs.
Specific Uses:
- Disease Modeling: hCiPSCs can be differentiated into various cell types to model human diseases and study disease mechanisms.
- Drug Discovery: hCiPSC-derived cells can be used for drug screening and development, providing a more relevant in vitro model for drug testing.
- Regenerative Medicine: hCiPSCs hold great promise for regenerative medicine, as they can potentially be used to generate replacement cells or tissues for transplantation.
- Basic Research: This kit is valuable for studying the mechanisms of cellular reprogramming and pluripotency.
Key Features and Benefits:
- Simple and Efficient: The kit offers a user-friendly process with high reprogramming efficiency.
- Versatility: Compatible with a variety of human somatic cell types.
- Safe and Non-Immunogenic: The chemical reprogramming approach avoids genetic manipulation, making it safer than traditional methods.
- Scalable: Can be used to generate large quantities of hCiPSCs for various applications.
- Defined Composition: The kit contains well-defined, serum-free media, ensuring consistent results.
In summary, The Human iPS Cell Chemical Reprogramming Kit is a valuable tool for researchers and scientists working in the fields of stem cell biology, disease modeling, drug discovery, and regenerative medicine. It offers a convenient and efficient way to generate hCiPSCs for a wide range of applications.
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Please Note: This Product's CoA, is not available. Please download the Date Sheet and MSDS below.
Human iPS Cell Chemical Reprogramming Kit
Introduction
Cellular reprogramming tools can manipulate cell identity, enabling the generation of desired cell types that provide broad applications in disease modeling, drug discovery and regenerative medicine. Chemical reprogramming approach synergistically targets cell signaling pathways and epigenetic modifiers without gene manipulation, and offers considerable advantages for the development of pharmacological and therapeutic strategies. Recently, a new scalable, efficient, and rapid chemical reprogramming approach was reported, which can induce human somatic cells into pluripotent stem cells (Guan, J et al., Nature, 2022; Liuyang, S et al., Cell Stem Cell, 2023). This kit, optimized from a patent in filing (METHODS FOR CHEMICAL REPROGRAMMING AND PLURIPOTENT STEM CELLS, PCT/CN2022/124960) and publication (Liuyang, S et al., Cell Stem Cell, 2023), can be used to generate human chemically induced pluripotent stem cell (hCiPSCs) from human adult adipose-derived stromal cells (hADSC) or skin dermal fibroblasts (HDFs).
Product Specifications
- Simple:
A user-friendly, easy-to-operate induction process which only involves 4 key steps by simply changing the medium as per the user's manual.
- Efficient:
Cell reprogramming efficiency ranges from 0.4% to 6.8% for skin fibroblasts and 1% to 31% for adipose derived stromal cells.
- Stable:
Efficient induction of somatic cells from diverse genetic back- grounds and various age groups.
- Easy Standardization:
Defined composition, serum-free and feed- er-independent, easy to standardize
- Safe:
The utilization of chemical small molecules avoids genome integration, non-immunogenic, safer than transgenic techniques.
Table: Kit contents (Cat# HST-crkit-001) (BCTCiPS-001)
Bottle | Label | Content | Function/ description |
1 | Medium A | 1 bottle, 100 mL | Medium for Stage 1 induction |
2 | Medium B | 1 bottle, 100 mL | Medium for Stage 2 induction |
3 | Medium C | 1 bottle, 30 mL | Medium for Stage 3 induction (Day 0~4) induction |
4 | Medium D | 1 bottle, 30 mL | Medium for Stage 3 induction (Day 4~8) induction |
5 | Medium E | 1 bottle, 30 mL | Medium for Stage 3 induction (Day 8~12) induction |
6 | Medium F | 1 bottle, 100 mL | Medium for hCiPS cell line derivation |
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Storage Conditions
The kit is shipped on dry ice. When placing an online order, it is mandatory to select Dry Ice shipping.
When stored at -15 to -25°C. Avoid repeated freezing and thawing. After thawing, store for up to 3 weeks at 2 to 8 °C .
Product Use
This product is designated for research purposes exclusively and is not intended for utilization in diagnostic or any other commercial applications. Multilateral Trade Agreements (MTA) dictate that you must refrain from engaging, either directly or indirectly, in the commercial utilization of these materials. This includes scenarios such as conducting research subject to consultancy or licensing obligations with third parties, screening compound libraries, or developing commercial products.
Reference
Guan, J., Wang, G., Wang, J., Zhang, Z., Fu, Y., Cheng, L., Meng, G., Lyu, Y., Zhu, J., Li, Y., et al. (2022). Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 605, 325-331.
Liuyang, S., Wang, G., Wang, Y., He, H., Lyu, Y., Cheng, L., Yang, Z., Guan, J., Fu, Y., Zhu, J., et al. (2023). Highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming. Cell stem cell 30, 450-459.e459.
Human iPS Cell Chemical Reprogramming Kit
- Product CAT#: HST-CRKit-001 (BCTCiPS-001)
- Availability: 2-3 Days
Available Options
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