PRODUCT DESCRIPTION

CellTran®PEI (PEI 40K, also known as PEI 22K in free base) is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs.

CellTran®PEI is easier to use and offers consistently higher titers than PEI 25K. PEI 25K transfection solutions typically take several hours to prepare, while CellTran®PEI can be converted to a ready-to-use solution in under two hours. Additionally, PEI 25K contains 4-11% residual propionyl groups, which prevents the polymer backbone from strongly binding to DNA. CellTran®PEI's fully depropionylated structure means each batch performs consistently higher.

FEATURES

• Do not effect the result with/without FBS. 
• Can be stored at RM/4°C.
• Easily use and save time. 

STABILITY & STORAGE

CellTran®PEI is stable within validity period when CellTran®PEI stored at Room temperature or 4 °C for at least 12 months.

DIRECTIONS FOR USE

• Cells preparation: Split 293T cells one day before transfection in DMEM/10% FBS medium:

• Prior to transfection bring all reagents to room temperature. In a sterile tube dilute total plasmid DNA (ug) in serum-free DMEM w/o phenol red. Add DNA and  PEI to the DMEM medium.dish medium (ul) DNA (ug) PEI (ug).

•  Room temperature for 15 Mins.
• Harvest transfected cells and/or viral supernatant at 48 hours post-transfection.