• Recombinant Human FLT3-Ligand

Product Specifications

  • Expression of Human Proteins in Human Cells
  • Extreme low Endotoxin
  • High Purity
  • Animal Free and Xeno Free
  • Tag Free
Source: Human cells derived
Structure: Glycosylated monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 24-30kDa

Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by its ability to stimulate the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Human Flt3 Ligand is a 24-30kDa glycoprotein with a 158 amino acid. Flt3 Ligand is expressed by T cells, bone marrow and thymic fibroblasts. The predominant biologically active form is membrane- bound isoform, which can be proteolytically cleaved to generate a biologically active soluble isoform. Flt3 Ligand synergizes well with a number of other colonies stimulating factors and interleukins to regulate proliferation of early hematopoietic cells by activating Flt3.

References

Lyman SD. et al.1995 Oncogene. 10 (1), 149-57.
Hannum C., et al. (1994) Nature 368,643-648.
Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
Stem Cells Studies
Description Hematopoietic stem cells culture
Documentations
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Recombinant Human FLT3-Ligand

  • CAT#: HHM-FT-0010 (10ug), HHM-FT-0100 (100ug), HHM-FT-1000 (1000ug)
  • Availability: In Stock
  • $0.00

  • Price in reward points: 50

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