Source: Mouse cells derived
Pysical Appearance: Liquid
Purity: >75% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 38-41kDa
Formulation:  0.2μm filtered solution in PBS, 1% CHAPS, 0.1%BSA

Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at -80°C recommended.

Activity Assay

Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a generate 100-fold increase of luciferase activity compared to control (buffer without Wnt3a). EC₅₀ is about 6 ng/mL.

Stability & Storage

Keep the protein frozen until use. Freeze at -20°C or below after thawed. Mix the protein by pipetting up and down only, but do not use vortexer.

Product Description

Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Mouse Wnt3a shows 96% amino acid identity to human Wnt3a protein.
This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.

References