Cell-Free Protein Synthesis: Fast, Flexible, and Powerful Protein Expression
A New Era of Rapid Protein Production
Traditional protein expression methods often take several days — from transformation and colony screening to culture and lysis.
Now, StemImmune’s new Cell-Free Protein Synthesis (CFPS) Kit revolutionizes this process, turning DNA directly into protein within hours — no living cells required.
Our E. coli-based T7 system retains the essential transcription and translation machinery (ribosomes, tRNAs, polymerases, cofactors), allowing researchers to perform fast, high-yield protein expression in vitro.
Whether you’re screening multiple constructs, expressing difficult targets, or producing proteins that are toxic to cells, CFPS provides unmatched speed and flexibility.
⚙️ How It Works
The Cell-Free Protein Synthesis Kit is a one-pot reaction containing:
- E. coli extract rich in ribosomes and translation factors
- Energy regeneration and amino acid mix
- T7 transcription system (DNA → mRNA → Protein)
- Buffer components optimized for stability and activity
Simply add your DNA or mRNA template — plasmid, linear PCR product, or transcribed RNA — and incubate.
Within 1–2 hours, protein synthesis begins, and most reactions reach optimal yield in 8–16 hours.
Why Cell-Free Protein Synthesis?
| Advantage | Description |
|---|---|
| Speed | Express proteins the same day — no need for transformation or culture. |
| Versatility | Works with plasmid, linear DNA, or mRNA templates. |
| Difficult Targets | Perfect for cytotoxic, insoluble, or disulfide-rich proteins. |
| High-Throughput Ready | Compatible with microplate or tube formats for screening and optimization. |
| Open System | Easily modify reactions by adding chaperones, cofactors, or non-canonical amino acids. |
Standard, Pro, and Max — Three Kit Options
StemImmune’s CFPS kits come in three optimized formulations:
| Kit | Key Feature | Typical Application |
|---|---|---|
| Standard | Up to 3 mg/mL yield | General-purpose protein expression |
| Pro | Chaperone-enriched | Insoluble or aggregation-prone proteins |
| Max | Redox-enhanced | Disulfide-rich, antibody fragments, cytokines |
Each kit is available in liquid or lyophilized formats for convenient storage and scalability.
Sample Performance Highlights
| Target Protein | Challenge | Result |
|---|---|---|
| Restriction Endonuclease | Insoluble in E. coli | Soluble, active enzyme in CFPS |
| Luciferase | Folding sensitive | Functional enzyme, strong luminescence |
| GFP-Nanobody | Aggregation-prone | Soluble and active binding |
| Fab/scFv | Disulfide-rich | Correctly folded, functional fragments |
| Cytokine (e.g., IL-2 variant) | Redox folding | Active protein via CFPS Max |
(Data derived from kit internal validation and sample performance testing.)
Ordering Information
| Kit Type | Size | Cat# |
|---|---|---|
| Standard (Liquid) | 20 rxns / 100 rxns | EG24301S / EG24301M |
| Standard (Lyophilized) | 20 rxns / 100 rxns | EG24302S / EG24302M |
| Pro (Liquid) | 20 rxns / 100 rxns | EG25301S / EG25301M |
| Max (Liquid) | 20 rxns / 100 rxns | EG25302S / EG25302M |
For quotes or technical inquiries, contact
info@stem-immune.com | 650-722-6613
About StemImmune LLC
Based in San Francisco Bay Area, StemImmune LLC develops advanced molecular biology reagents and cell-based solutions for biotechnology and therapeutic research.
From cell line development and protein expression to chromosome analysis, we provide reliable, human-cell-derived tools for precision life-science applications.
Summary
StemImmune’s Cell-Free Protein Synthesis Kit offers researchers a fast, flexible, and powerful alternative to traditional in-cell expression systems.
By enabling direct DNA-to-protein synthesis within hours, CFPS accelerates discovery — ideal for enzyme engineering, structural biology, antibody development, and translational research. For more information please visit Cell Free Protein.
