Product Specifications

• Expression of Human Proteins in Human Cells
• Extreme low Endotoxin
• High Purity
• Animal Free and Xeno Free
• Tag Free

Source: Human cells derived
Structure: Non-glycosylated homodimer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 29-35kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured   by   its   anti- virus function in A549 cells (human lung adenocarcinoma epithelial cell line) infected with encephalomyocarditis (EMC) virus.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

IL-29 belongs to the type III interferon protein family, which is distinct from the type I IFNs. IL- 29 is induced by virus infection or double stranded RNA. IL-29 acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state.  IL-29 has antiviral, antitumour and immunomodulatory activities. IL-29 is primarily active in epithelial cells and this cell type selective action is because of the epithelial cell specific expression of its receptor IFNLR1. It also exerts an immunomodulatory effect by up regulating MHC class I antigen expression.

References

Sheppard P, et al. (2003) Nat. Immunol. 4, 63-68.
Donnelly RP, et al. (2010) J. Interferon Cytokine. Res. 30,555-564.
Kotenko SV, et al. (2003) Nat. Immunol. 4, 69-77.