Product Application: Recombinant Human FLT3-Ligand

Primary Application:

Hematopoietic Stem Cell Expansion: FLT3-Ligand is a critical growth factor for the expansion of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells.
Immunotherapy: FLT3-Ligand can be used to generate dendritic cells (DCs) and other immune cells for immunotherapy applications.
Stem Cell Research: It is a valuable tool for studying hematopoiesis and stem cell biology.

Specific Uses:

Bone Marrow Transplantation: FLT3-Ligand can be used to mobilize hematopoietic stem cells from the bone marrow into the peripheral blood, facilitating bone marrow transplantation procedures.
Cancer Therapy: It can be used in combination with other therapies to stimulate hematopoiesis and reduce the side effects of chemotherapy.
Research: FLT3-Ligand is widely used in research to study hematopoiesis, immune cell development, and stem cell biology.

Key Features and Benefits:

High Purity and Low Endotoxin: Ensures optimal biological activity and minimal off-target effects.
Recombinant Human FLT3-Ligand: Ensures consistent quality and activity.
Easy to Use: Lyophilized format allows for easy storage and reconstitution.
Versatile: Can be used in a variety of applications, including research, clinical, and therapeutic settings.

In summary, Recombinant Human FLT3-Ligand is a valuable tool for researchers and clinicians working in the fields of hematology, immunology, and stem cell biology. It offers a versatile and effective way to manipulate hematopoietic cell development and function.

Citations for Recombinant Human FLT3-Ligand

Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.

Product Specifications

Expression of Human Proteins in Human Cells
Extreme low Endotoxin
High Purity
Animal Free and Xeno Free
Tag Free

Source: Human cells derived
Structure: Glycosylated monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 24-30kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by its ability to stimulate the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

FLT3L purity and Activity Assay

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Human Flt3 Ligand is a 24-30kDa glycoprotein with a 158 amino acid. Flt3 Ligand is expressed by T cells, bone marrow and thymic fibroblasts. The predominant biologically active form is membrane- bound isoform, which can be proteolytically cleaved to generate a biologically active soluble isoform. Flt3 Ligand synergizes well with a number of other colonies stimulating factors and interleukins to regulate proliferation of early hematopoietic cells by activating Flt3.