Product Application: Recombinant Mouse Wnt3A
Primary Application:
- Stem Cell Research: Wnt3a is essential for maintaining the pluripotency of stem cells.
- Developmental Biology: Wnt3a plays a crucial role in embryonic development, including cell fate determination and tissue patterning.
- Cancer Research: Wnt3a signaling is involved in cancer development and progression.
- Regenerative Medicine: Wnt3a can be used to promote tissue regeneration and repair.
Specific Uses:
- Stem Cell Culture: Wnt3a is used to culture and maintain stem cells.
- Drug Discovery: Wnt3a can be a target for drug development, particularly in the areas of cancer and regenerative medicine.
- Research: Wnt3a is widely used in research to study cell signaling, development, and disease.
Key Features and Benefits:
- High Purity and Low Endotoxin: Ensures optimal biological activity and minimal off-target effects.
- Recombinant Mouse Wnt3a: Guarantees consistent quality and activity.
- Versatile: Can be used in a variety of applications, including research, drug development, and therapeutic interventions.
In summery, Recombinant Mouse Wnt3a is a crucial signaling molecule involved in various biological processes, including stem cell maintenance, embryonic development, cancer progression, and tissue regeneration. It is widely used in stem cell research, developmental biology, cancer research, and regenerative medicine. Researchers use it to study cell signaling, development, and disease mechanisms, while drug developers target it for cancer and regenerative medicine therapies.
Citations for Recombinant mouse Wnt3a
- Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment. Hepatology. 2022 Mar 1.doi: 10.1002
- Single cell Raman spectroscopy to identify different stages of proliferating human hepatocytes for cell therapy. Stem Cell Res Ther. 2021 Oct 30;12(1):555.
- In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity. Cell Stem Cell. Volume 23, Issue 6, 6 December 2018, Pages 806-819.e4
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Source: Mouse cells derived
Pysical Appearance: Liquid
Purity: >75% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 38-41kDa
Formulation: 0.2μm filtered solution in PBS, 1% CHAPS, 0.1%BSA
Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at -80°C recommended.
Activity Assay
Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a generate 100-fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.

Stability & Storage
Keep the protein frozen until use. Freeze at -20°C or below after thawed. Mix the protein by pipetting up and down only, but do not use vortexer.
Product Description
Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Mouse Wnt3a shows 96% amino acid identity to human Wnt3a protein.
This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
References
Taranjit S. Gujral, et al, (2014). Cell. 159: 844-856.
Kahn.M (2014) Nature Reviews Drug Discovery 13: 513-532.
Recombinant Mouse Wnt3a
- Brand: StemImmune LLC
- Product CAT#: MST-WT3a-001 (1ug), MST-WT3a-002 (2ug), MST-WT3a-0010 (10ug)
- Availability: In Stock
Available Options
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Tags: Growth Factors