Product Application: Recombinant Murine LIF (E. coli Derived)
Primary Application:
- Stem Cell Research: LIF is essential for maintaining the pluripotency of embryonic stem cells.
- Neurobiology: LIF plays a role in neuronal development and neuroprotection.
- Immunology: LIF can modulate the immune response and inflammation.
- Tissue Engineering: LIF can be used to promote cell proliferation and differentiation in tissue engineering applications.
Specific Uses:
- Stem Cell Culture: LIF is used in the culture and maintenance of embryonic stem cells.
- Drug Discovery: LIF can be a target for drug development, particularly in the areas of neurodegenerative diseases and regenerative medicine.
- Research: LIF is widely used in research to study cell differentiation, neurobiology, and immunology.
Key Features and Benefits:
- High Purity and Low Endotoxin: Ensures optimal biological activity and minimal off-target effects.
- Recombinant Murine LIF: Guarantees consistent quality and activity.
- Easy to Use: Lyophilized format allows for easy storage and reconstitution.
- Versatile: Can be used in a variety of applications, including research, drug development, and tissue engineering.
In summery, Recombinant Murine LIF is a crucial cytokine for maintaining stem cell pluripotency, neuronal development, and immune modulation. It is widely used in stem cell research, neurobiology, and immunology studies. In addition, it has potential applications in drug discovery and tissue engineering. While E. coli-derived LIF is a cost-effective option, it's important to consider potential differences in glycosylation compared to mammalian cell-derived LIF, which may impact its biological activity.
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Source: E.coli derived
Structure: Monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <1EU/ug
Molecular Weight: 20kDa (monomer)
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein
Activity Assay
The activity was measured by M1 cell differentiation assay. ED50 for this effect is typically 0.5 ng/mL.
Reconstitution
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Protein Description
LIF is a pleiotropic member of the IL6 family of cytokines produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF functions through a heterodimeric receptor complex containing a ligand binding subunit, LIF Rlex containing a ligand and melanoma with complex effects. LIF either induces or inhibits differentiation in different cell environments. LIF promotes long-term maintenance of pluripotency in embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of protein synthesis by hepatocytes, and differentiation of cholinergic nerves acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes.
References
Metcalfe, S.M. (2011) Genes Immun. 12:157.
Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
Yue X, et al. (2015) Cancer Cell Microenviron. 2015;2(3). pii: e877.
Recombinant Murine LIF
- Brand: StemImmune LLC
- Product CAT#: EST-mLIF-0010(10ug), EST-mLIF-0100(100ug), EST-mLIF-1000(1000ug)
- Availability: In Stock
Available Options
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Tags: Growth Factors