Product Application: Recombinant Human Wnt5A
Primary Application:
- Developmental Biology: Wnt5a plays a crucial role in various developmental processes, including cell fate determination, tissue patterning, and organogenesis.
- Cancer Research: Wnt5a is involved in cancer development and progression.
- Stem Cell Research: Wnt5a can influence stem cell differentiation and proliferation.
Specific Uses:
- Drug Discovery: Wnt5a can be a target for drug development, particularly in the areas of cancer and regenerative medicine.
- Research: Wnt5a is widely used in research to study cell signaling, development, and disease.
Key Features and Benefits:
- High Purity and Low Endotoxin: Ensures optimal biological activity and minimal off-target effects.
- Recombinant Human Wnt5a: Guarantees consistent quality and activity.
- Versatile: Can be used in a variety of applications, including research, drug development, and therapeutic interventions.
In summery, Wnt5a is a crucial signaling molecule involved in development, cancer, and stem cell biology. It's valuable for research and drug discovery. High purity, low endotoxin, and recombinant origin ensure quality and activity.
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Product Specifications
- Expression of Mouse Cells
- Extreme low Endotoxin
- High Purity
- Tag Free
Source: CHO cells derived
Physical Appearance: Liquid
Purity: >75% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: ~45kDa
Formulation: PBS pH7.4-7.6, 1%Chaps, 0.1% BSA
Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at -80°C recommended.
Activity Assay
The inhibitory activity of Wnt5a on the canonical Wnt pathway has been measured using TCF- based Wnt reporter stable cell line stimulated by mouse Wnt3a. IE50 of Wnt5a is about 50-100 ng/mL in the presence of 1-2 ng/mL of mouse Wnt3a.
Stability & Storage
Keep the protein frozen until use. Freeze at -20°C or below after thawed. Mix the protein by pipetting up and down only, but do not use vortexer.
Product Description:
The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. Protein Wnt5a is a protein that is encoded by the WNT5A gene. This gene is a member of the WNT gene family. Mature mouse Wnt5a is 100% identical in amino acids to mature human Wnt5a after being secreted from expression cells. Wnt5a protein can inhibits or activates TCF-based Wnt signaling. This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound Sepharose beads, and followed by gel filtration.
References
Bauer M, et al. PLoS ONE 8(11): e80526.
Janda CY, et al. (2012) Science.337(6090): 59-64.
Milkels AJ, et al. (2006) PLoS Biol, 4: e115.
Recombinant Human/Mouse WNT5A
- Brand: StemImmune LLC
- Product CAT#: HST-WT5a-0001 (1ug), HST-WT5a-0002 (2ug), HST-WT5a-0010 (10ug)
- Availability: In Stock
Available Options
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Tags: Growth Factors