Product Application: Recombinant Proteinase K
Primary Application:
- Protein Digestion: Proteinase K is used to digest proteins into smaller peptides for various applications.
- Molecular Biology: It is used in DNA and RNA isolation to remove proteins and other contaminants.
- Biomedical Research: It is used in various research applications, such as Western blotting, protein sequencing, and protein purification.
Specific Uses:
- DNA and RNA Isolation: To remove proteins from DNA and RNA samples.
- Protein Digestion: To generate peptides for mass spectrometry analysis.
- Western Blotting: To remove proteins from membranes before antibody staining.
- Protein Purification: To remove unwanted proteins from protein samples.
Key Features and Benefits:
- High Purity and Activity: Ensures efficient protein digestion.
- Broad Substrate Specificity: Digests a wide range of proteins.
- Stability: Maintains activity under various conditions.
- Easy to Use: Simple and straightforward digestion protocols.
In summery, Recombinant Proteinase K is a versatile enzyme used for protein digestion in various applications. It is particularly useful in molecular biology for DNA and RNA isolation, where it efficiently removes proteins and other contaminants. In biomedical research, it finds applications in techniques like Western blotting, protein sequencing, and purification. Its broad substrate specificity, stability, and ease of use make it a valuable tool in many laboratory settings.
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Source: Yeast derived
Purity: >98% by SDS-PAGE
Endotoxin Level: N/O
Molecular Weight: 28.93kDa
Activity Assay
Proteinase K activity can be measured using hemoglobin as the substrate
Description
Proteinase K is a serine proteas and belongs to Peptidase family S8. This enzyme has been widely exhibits substrate specificity and degrades many proteins in the native state. Proteinase K is inhibited by serine protease inhibitors such as phenylmethylsulfonyl fluoride. This enzyme activity is unaffected by the sulfhydryl modifying reagents. It is stable in EDTA buffer.
Reconstitution
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Proteinase K Stock Solution - 20 mg/ml (Download PDF format)
Instructions
1) Tris-CaCl2 buffer PH8.0 (200mM Tris PH8.0+3mM CaCl2)
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Measure 5 ml of 200mM Tris Buffer (pH 8.0), RNase free.
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Add 1.66 mg (3mM) calcium chloride (CaCl2) to the Tris Buffer and dissolve.
2) 5ml Stock Solution (20mg/ml)
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Measure 2.5 ml of the Tris-CaCl2 Buffer (pH 8.0) into a centrifuge tube.
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Add 100 mg Proteinase K (YPK-K-100GM).
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Mix gently to dissolve the Proteinase K.
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Sterilize the solution with a 0.22 μm filter.
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Fill to a final volume of 5 ml with 100% glycerol (add 2.5ml glycerol).
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Mix until completely dissolved.
Store in aliquots at -20°C for 1 year.
3) Suggested Work Concentration: 0.05~1mg/ml
Note: Proteinase K interacts with nucleases. To ensure maximum Proteinase K activity, use water that has been treated with 0.1% DEPC
Recombinant Proteinase K
- Product CAT#: YPE-K-1G (1g), YPE-K-5G (5ug), YPE-K-100G (100g)
- Availability: In Stock
Available Options
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Tags: Proteinase