Restriction Enzymes

LightNing® fast enzymes deliver 5–15 min digests with a single universal buffer, designed for single, double, or triple digests while keeping very low star activity. The optional CutOne® Color buffer lets you load gels directly after digestion.

Why LightNing®

Fast workflow: typical reactions finish in 5–15 minutes.
Universal buffer simplifies single/double digests and downstream steps.
Very low star activity even after long incubations.
Color buffer variant supports direct gel loading.
ADCF (animal-derived-component-free) and GMP Grade options available.

Quick Setup (20 µl)

DNA ≤1 µg, 2 µl 10× buffer (or Color buffer), enzyme 1 µl, water to 20 µl; 37 °C for 5–15 min.

Heat-inactivation per enzyme spec if required.

Good to Know

Use the universal buffer for both single and double digests; color buffer includes tracking dyes.

ADCF = animal-derived-component-free; GMP line is produced with no animal-origin components.

BsrGI

BsrGI (Restriction Enzyme) REF: EG23505S Recognition & Cleavage Recognition site:..

DpnI

DpnI (Type IIM Restriction Enzyme) REF: EG15585S Recognition & Cleavage Recogniti..

DpnII

DpnII (Restriction Enzyme) REF: EG15533S Recognition & Cleavage Recognition site:..

EcoRI

EcoRI (Restriction Enzyme) REF: EG15536S Recognition & Cleavage Recognition site:..

EcoRV

EcoRV (Restriction Enzyme) REF: EG15537S Recognition & Cleavage Recognition site:..

Esp3I (BsmBI)

Esp3I (BsmBI, Type IIS Restriction Enzyme) REF: EG21503S Recognition & Cleavage R..

HindIII

HindIII (Restriction Enzyme) REF: EG15539S Recognition & Cleavage Recognition sit..

KpnI

KpnI (Restriction Enzyme) REF: EG15544S Recognition & Cleavage Recognition site: ..

MluI

MluI (Restriction Enzyme) REF: EG15547S Recognition & Cleavage Recognition site: ..

NcoI

NcoI (Restriction Enzyme) REF: EG15550S Recognition & Cleavage Recognition site: ..

NdeI

NdeI (Restriction Enzyme) REF: EG15551S Recognition & Cleavage Recognition site: ..

NheI

NheI (Restriction Enzyme) REF: EG15552S Recognition & Cleavage Recognition site: ..

NotI

NotI (Restriction Enzyme) REF: EG15553S Recognition & Cleavage Recognition site: ..

NsiI

NsiI (Restriction Enzyme) REF: EG15606S Recognition & Cleavage Recognition site: ..

PacI

PacI (Restriction Enzyme) REF: EG15603S Recognition & Cleavage Recognition site: ..

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