Product Application: BeiCell Human Chemical Reprogramming Kit (2nd Generation)

Primary Application:
Generation of Human Chemically Induced Pluripotent Stem Cells (hCiPSCs): This 2nd-generation kit enables the rapid, efficient, and stable reprogramming of human somatic cells (including fibroblasts, adipose-derived stromal cells, and umbilical cord mesenchymal cells) into hCiPSCs without genetic modification.

Specific Uses:

Disease Modeling: hCiPSCs can be differentiated into diverse cell types for modeling human diseases and studying disease mechanisms.
Drug Discovery: hCiPSC-derived cells provide a reliable platform for high-throughput drug screening, toxicity testing, and mechanism studies.
Regenerative Medicine: hCiPSCs hold promise for cell and tissue replacement therapies in future translational applications.
Basic Research: The kit offers a valuable system for investigating the mechanisms of chemical reprogramming, pluripotency induction, and epigenetic regulation.

Key Features and Benefits:

Fast & Efficient: Shortens reprogramming time from ~30 days to as little as 10–16 days.
High Induction Efficiency: Achieves up to 38% efficiency across diverse donor cells.
Versatile: Effective for somatic cells from different genetic backgrounds and ages.
Safe & Non-Immunogenic: Small molecules avoid genome integration, lowering risks compared to viral or transgenic methods.
Standardized & Defined: Serum-free, feeder-free, well-defined composition ensures reproducibility and scalability.

In summary, the BeiCell Human Chemical Reprogramming Kit (2nd Generation) is a powerful tool for stem cell researchers, enabling rapid, safe, and efficient generation of hCiPSCs. It supports a wide range of applications from basic biology to disease modeling, drug discovery, and regenerative medicine research.

BeiCell Human Chemical Reprogramming Kit (2nd Generation)

Efficient and Stable Cell Reprogramming

BeiCell Human Chemical Reprogramming Kit (2nd Generation) is a fast, efficient, and stable tool for researchers in cell therapy. It supports reprogramming of mesenchymal cells or fibroblasts from adipose tissue, skin, umbilical cord, and other tissues.

The kit builds on breakthroughs from Prof. Deng Hongkui’s team at Peking University (Nature, 2022; Cell Stem Cell, 2023; Nature Chemical Biology, 2025).

BeiCell chemical reprogramming kit overview

It enables stable, highly efficient induction of human cells into human chemically induced pluripotent stem cells (CiPS) with no transgenic manipulation. Small molecules permeate cells and precisely regulate state, achieving reprogramming through a simple, highly safe process—an independently developed next-generation human pluripotent stem cell technology following nuclear transfer and transcription-factor overexpression.

Latest Breakthrough in Chemical Reprogramming

Published on January 3, 2025 in Nature Chemical Biology by teams led by Prof. Deng Hongkui and Prof. Guan Jingyang, the optimized rapid chemical reprogramming system identified key epigenetic barriers and reduced induction time from ~30 days to under 16 days (as fast as 10 days).

Across donors from 15 individuals, CiPS induction succeeded in all cases, reaching a maximum efficiency of 38%. For previously hard-to-reprogram cells, efficiency improved by >20× within 16 days, broadening applicability across diverse genetic backgrounds and ages.

Technical Advantages

Simple Operation: Reprogramming by straightforward medium exchanges.
High Induction Efficiency: Completion within ~20 days; up to 38% efficiency.
Broad Applicability: Robust across donors with different backgrounds and ages.
Safe & Reliable: Small molecules do not integrate into the genome; lower immunogenic risk.
Standardized Production: Well-defined, serum-free and feeder-free components.

Human CiPS Cell Preparation Process

Chemical reprogramming is a multi-stage, continuous culture process—no viral/plasmid vectors or electroporation required. Conversion to CiPS cells is achieved via simple medium changes, morphological observation, and passaging (see Figure 1).

Human CiPS chemical reprogramming process diagram

Key Steps

Seeding of Initial Cells (Initial Cell Seeding Medium)
First Stage Induction (Medium A)
Second Stage Induction (Medium B)
Third Stage Induction (Media C, D, E)
Single-Cell Passaging Culture (Medium F)
Human CiPS Cell Expansion Culture (Human CiPS Cell Culture Medium)

Throughout induction, adipose/skin/umbilical-cord–derived mesenchymal or fibroblast cells pass through three media-defined phases. Progress is monitored by morphology and immunofluorescence markers.

Morphological changes during chemical reprogramming

Efficiency and colony formation of CiPS cells

Immunofluorescence detection of key protein markers

Kit Components and Storage

* See Table 1 for detailed composition. ** Research Use Only; not for clinical diagnosis or treatment.

Storage Conditions

Thaw frozen medium overnight at 4 °C or at room temperature (precipitates/flocculence can be normal).
After thawing, store at 4 °C for up to 2 weeks or aliquot at −20 °C for up to 1 year (avoid repeated freeze–thaw).

References

1. Wang, Y. et al. A rapid chemical reprogramming system to generate human pluripotent stem cells. Nat. Chem. Biol. (2025). DOI: 10.1038/s41589-024-01799-8.

2. Liuyang, et al. Highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming. Cell Stem Cell 30, 450-459.e9 (2023).

3. Guan, et al. Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 605, 325–331 (2022).