SalI (Restriction Enzyme)

REF: EG15566S

Recognition & Cleavage

Recognition site: 5'-GTCGAC-3' (palindromic)

Cleavage pattern:

5' ... G^▼T C G A C ... 3'
3' ... C A G C T_▲G ... 5'

Type: II restriction endonuclease · Common use: Routine cloning and fast DNA digestion

Isoschizomers: Sfr274I, SalGI (methylation sensitivities may differ)

Specs

Optimal temperature: 37 °C
Fast digest: 5–15 min in 1× CutOne® / CutOne® Color Buffer
Heat inactivation: 80 °C · 20 min
Storage: −20 °C

Pack Components

Component	EG15566S
SalI (20 U/µl)	200 µl (4000 U)
10× CutOne® Buffer	2 × 1 ml
10× CutOne® Color Buffer	2 × 1 ml

Recommended Reaction

Buffer: 1× CutOne® or 1× CutOne® Color Buffer
Incubation: 37 °C
Typical duration: 15 min (plasmid); 15–30 min (PCR product); 30–60 min (genomic DNA)

CutOne® Color Buffer includes density reagent and red/yellow dyes for direct gel loading.

Quick Protocol (Fast DNA Digestion)

20 µl (plasmid): 15 µl ddH₂O, 2 µl 10× CutOne®, ≤1 µg DNA, 1 µl SalI.
30 µl (PCR product): 16 µl ddH₂O, 3 µl 10× CutOne®, ~0.2 µg DNA, 1 µl SalI. If PCR not purified, reduce buffer to 2 µl.
50 µl (genomic DNA): 30 µl ddH₂O, 5 µl 10× CutOne®, 5 µg DNA, 5 µl SalI.
Mix gently, quick-spin; incubate at 37 °C for the indicated time.
(Optional) Heat-inactivate at 80 °C for 20 min or load directly (Color Buffer).

Notes

Total enzyme ≤10% of reaction (limit glycerol to avoid star activity).
DNA contaminants (phenol, ethanol, EDTA, salt, detergents) inhibit digestion.
Increase incubation time if volume >20 µl.

Quality Control

Functional test: 1 µg λDNA + 1 µl SalI → complete digest in 15 min at 37 °C (20 µl).
Prolonged incubation/star activity: 3 h at 37 °C shows no nuclease degradation; longer may cause star activity.
Ligation & recutting: >95% ligation at 22 °C (T4 DNA ligase); >95% fragments recut.
Non-specific endonuclease: <10% nicked/linear after 4 h at 37 °C.
Blue/White assay: <1% white colonies (ends intact).

Methylation Effects

Methylase	Effect
Dam	No effect
Dcm	No effect
CpG	Some cleavage blocked
EcoKI	No effect
EcoBI	No effect

Recognition Sites in Common DNAs

DNA	# Sites	DNA	# Sites
λDNA	2	ΦX174	0
pBR322	1	pUC57	1
pUC18/19	1	SV40	0
M13mp18/19	1	Adeno2	3

Activity in Different Buffers

Buffer	Activity
CutOne®	100%
Thermo FastDigest	100%
NEB rCutSmart™	100%
Takara QuickCut™	100%

*Activities determined under functional test conditions.

Write a review

Your Name

Your Review

Note: HTML is not translated!

Rating Bad Good

Captcha

Please complete the captcha validation below

SalI

Brand: Best Enzymes
Product CAT#: EG15566S
Availability: In Stock

Available Options

Enzymes Handling Fee

Overnight Shipping Handling (+$29.99)

Size

Qty

0 reviews / Write a review