Storage Condition

Store at 4℃ for 6 months. For long-term storage, keep at −20℃.

Components

Description

FY2000 DNA Marker contains 6 DNA fragments: 100, 250, 500, 750, 1000, and 2000 bp. Each 5 μl load provides ~50 ng DNA per band, except the 750 bp band which is ~120 ng for enhanced visibility.

Supplied in 1× loading buffer with tracking dyes, ready to use. Bands are designed with uniform intensity for clear resolution and approximate DNA quantification.

Protocol

1. Load 5 μl of FY2000 DNA Marker per lane (adjust based on comb size).
2. Run in 1.0–2.0% agarose gel (REF: EG20910) with 1× TAE buffer at 5–10 V/cm. Always use fresh gel and buffer.
3. Stain with Safe Red DNA Stain (REF: CP18106S) or other nucleic acid dyes; visualize under UV.
4. For best results, ensure the bromophenol blue tracking dye migrates to ~2/3 of gel length.

Notice

• Premixed with buffer and dyes; load directly without dilution.
• Stable at room temperature during routine use.
• Avoid repeated freeze–thaw cycles; aliquot before storage.