• Recombinant Human WNT3A

Product Application: Recombinant Human Wnt3a

Primary Application:

  • Stem Cell Research: Wnt3a is essential for maintaining the pluripotency of stem cells.
  • Developmental Biology: Wnt3a plays a crucial role in embryonic development, including cell fate determination and tissue patterning.
  • Cancer Research: Wnt3a signaling is involved in cancer development and progression.
  • Regenerative Medicine: Wnt3a can be used to promote tissue regeneration and repair.

Specific Uses:

  • Stem Cell Culture: Wnt3a is used to culture and maintain stem cells.
  • Drug Discovery: Wnt3a can be a target for drug development, particularly in the areas of cancer and regenerative medicine.
  • Research: Wnt3a is widely used in research to study cell signaling, development, and disease.

Key Features and Benefits:

  • High Purity and Low Endotoxin: Ensures optimal biological activity and minimal off-target effects.
  • Recombinant Human Wnt3a: Guarantees consistent quality and activity.
  • Easy to Use: Easy storage and reconstitution.
  • Versatile: Can be used in a variety of applications, including research, drug development, and therapeutic interventions.

In summery, Recombinant Human Wnt3a is a crucial signaling molecule involved in various biological processes, including stem cell maintenance, embryonic development, cancer, and tissue regeneration. It's a valuable tool for research, drug discovery, and therapeutic applications. High purity, low endotoxin, and recombinant origin ensure quality and activity. The lyophilized format offers convenience.

Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.

 

Product Specifications

  • Extreme low Endotoxin
  • High Purity
  • Tag Free

Source: CHO cells derived
Purity: >70% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 38-41kDa
Formulation: PBS PH7.4-7.6, 1% Chaps, 0.1% BSA.

Activity Assay

Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line10 ng/mL of Wnt3a generate 50-fold increase of luciferase activity compared to control (buffer without Wnt3a).

Activity and Purity

Reconstitution

Briefly centrifuge the vial before opening.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Product Description:

Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Human Wnt3a shows 96% amino acid identity to mouse Wnt3a protein.
This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound Sepharose beads, and followed by gel filtration.

References

Lu, D and et al. (2011) Oncotarget. 2: 587 - 587.
Taranjit S. Gujral, et al, (2014). Cell. 159: 844-856.
Kahn.M (2014) Nature Reviews Drug Discovery 13: 513–532.

Write a review

Note: HTML is not translated!
    Bad           Good
Captcha

Recombinant Human WNT3A

  • Brand: StemImmune LLC
  • Product CAT#: HST-WT3a-0001 (1ug), HST-WT3a-0002 (2ug), HST-WT3a-0010 (10ug)
  • Availability: In Stock

Available Options


Related Products

Recombinant Mouse Wnt3a

Recombinant Mouse Wnt3a

Source: Mouse cells derived Pysical Appearance: Liquid Purity: >75% by SDS-PAGE Endotoxin Leve..

$0.00

Recombinant Human/Mouse WNT5A

Recombinant Human/Mouse WNT5A

Product Specifications Expression of Mouse Cells  Extreme low Endotoxin  High Pu..

$0.00

Tags: Growth Factors