Product Application: Proteinase K Ready-to-Use
Primary Application:
- Protein Digestion and Purification: Proteinase K is widely used for the complete digestion of proteins, which is essential for various applications, such as protein purification, mass spectrometry, and peptide analysis.
Specific Uses:
- Protein Purification: It can be used to remove contaminants from protein samples, improving the purity of the target protein.
- Western Blotting: Proteinase K can be used to digest unwanted proteins in lysates before Western blotting, reducing background noise and improving signal clarity.
- Mass Spectrometry: It is used to prepare protein samples for mass spectrometry analysis by digesting them into smaller peptides.
- Peptide Synthesis: Proteinase K can be used to cleave synthetic peptides at specific sites, generating fragments for further analysis or modification.
- DNA Extraction: Proteinase K is commonly used to degrade proteins during DNA extraction, preventing protein contamination of the DNA sample.
Key Features and Benefits:
- Broad Substrate Specificity: Digests a wide range of proteins, making it versatile for various applications.
- Native State Digestion: Can digest proteins in their native, folded state, simplifying sample preparation.
- Heat Stability: Remains active at elevated temperatures, allowing for efficient digestion under various conditions.
- EDTA Stability: Can be used in buffers containing EDTA without affecting its activity.
- Ready-to-Use Format: Convenient for immediate use, saving time and effort.
In summary, Proteinase K Ready-to-Use is a valuable tool for researchers and scientists working in a variety of fields, including biochemistry, molecular biology, and proteomics. Its broad substrate specificity, heat stability, and ease of use make it an essential enzyme for protein digestion and purification applications.
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Proteinase K Ready-to-Use Solution
Yeast-Derived • High Purity • Molecular Biology Grade
Overview
StemImmune Proteinase K Ready-to-Use Solution is supplied at 20 mg/mL and is designed for convenient use in DNA extraction, RNA extraction, protein digestion, and molecular biology workflows. Proteinase K is a broad-spectrum serine protease capable of degrading a wide variety of proteins, including native and denatured proteins.
Product Information
| Product Name | Proteinase K Ready-to-Use Solution |
| Source | Yeast-derived |
| Purity | >98% by SDS-PAGE |
| Molecular Weight | 28.93 kDa |
| Format | Ready-to-Use Solution |
| Concentration | 20 mg/mL |
| Activity | >600 mAU/mL |
| Endotoxin Level | N/O |
| Storage | 15–25°C |
| Catalog Numbers | YPE-PK-001 (1 mL) YPE-PK-010 (10 mL) |
Applications
- DNA Extraction
- RNA Extraction
- Protein Digestion
- Genomic DNA Purification
- Removal of Protein Contaminants
- PCR and Molecular Biology Sample Preparation
- Cell and Tissue Lysis Workflows
Activity Assay
Proteinase K activity can be measured using hemoglobin as the substrate.
Description
Proteinase K belongs to the serine protease family (Peptidase S8) and exhibits broad substrate specificity. Unlike many proteases, Proteinase K efficiently digests proteins in their native folded conformation, making it highly effective for nucleic acid purification and protein removal.
Proteinase K activity can be inhibited by serine protease inhibitors such as PMSF. The enzyme remains stable in buffers containing EDTA and is generally unaffected by sulfhydryl-modifying reagents.
Suggested Working Concentration
Recommended working concentration: 0.05–1 mg/mL depending on sample type and application.
Reconstitution
StemImmune Ready-to-Use Proteinase K does not require reconstitution and can be used directly from the bottle.
Stability & Storage
Store at 15–25°C. Avoid contamination during use. Refer to the Certificate of Analysis for lot-specific information.
Note: For dilution, use nuclease-free water. DEPC-treated water may be used when appropriate for RNA-related applications.
Frequently Asked Questions
What is Proteinase K used for?
Proteinase K is commonly used for DNA extraction, RNA extraction, protein digestion, and removal of protein contaminants from nucleic acid samples.
Does StemImmune Proteinase K require reconstitution?
No. It is supplied as a ready-to-use solution at 20 mg/mL.
What is the recommended working concentration?
Typical working concentrations range from 0.05–1 mg/mL depending on the application.
Can Proteinase K be used for genomic DNA purification?
Yes. Proteinase K is widely used in genomic DNA isolation and purification workflows.
Proteinase K (Ready-to-Use)
- Product CAT#: YPE-PK-001 (ml), YPE-PK-010 (10ml)
- Availability: In Stock
Available Options
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