Product Application: Recombinant Human Vitronectin (E. coli Derived)
Primary Application:
- Cell Culture: Vitronectin is a critical component of cell culture media, promoting cell adhesion and proliferation.
- Tissue Engineering: Vitronectin can be used to create biomaterials and scaffolds for tissue engineering.
- Wound Healing: Vitronectin plays a role in wound healing by promoting cell migration and proliferation.
- Stem Cell Research: Vitronectin is used to culture and maintain stem cells, including embryonic stem cells and induced pluripotent stem cells.
Specific Uses:
- Cell Culture: Vitronectin is used to coat culture plates and other surfaces to improve cell attachment and growth.
- Tissue Engineering: Vitronectin can be incorporated into biomaterials to enhance cell adhesion and tissue formation.
- Research: Vitronectin is widely used in research to study cell adhesion, migration, and differentiation.
Key Features and Benefits:
- High Purity and Low Endotoxin: Ensures optimal biological activity and minimal off-target effects.
- Recombinant Human Vitronectin: Guarantees consistent quality and activity.
- Easy to Use: Lyophilized format allows for easy storage and reconstitution.
- Versatile: Can be used in a variety of applications, including research, cell culture, and tissue engineering.
In summery, Recombinant Human Vitronectin is a crucial extracellular matrix protein that promotes cell adhesion, proliferation, and differentiation. It's essential for cell culture, tissue engineering, wound healing, and stem cell research. High purity, low endotoxin, and recombinant origin ensure quality and activity. The lyophilized format offers convenience.
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Please Note: This Product's CoA, Data Sheet and SDS are not available.
Source: E. Coli derived
Purity: >95% by SDS-PAGE
Structure: Monomer
Endotoxin Level: <0.1EU/ug
Molecular Weight: ~62kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein
Activity Assay
Recombinant Human Vitronectin promotes attachment of adherent cell in serum free, such as hESC and iPSC. Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells, when 5 x 104 cells/well are added to Vitronectin coated plates (5 µg/mL with 100 µL/well), approximately >60% will adhere after 30 minutes at 37 °C.
Reconstitution
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Protein Description
Vitronectin is a secreted glycoprotein detected as a mixture of 75 kilodalton (kDa) and 65 kDa polypeptides in serum and the extracellular matrix. It primarily in monomeric form, but it can also be found as a dimer after endogenous cleavage. Vitronectin can support cell adhesion and spreading through binding to various integrins and other proteoglycans. The cell-binding site of vitronectin involves a common cell-attachment tripeptide Arg-Gly-Asp Additionally, recombinant vitronectin can function as a chemically-defined matrix component in human embryonic stem cell and induced pluripotent stem cell. The full length of recombinant human Vitronectin with His tag expressed in E coli migrates at an apparent molecular weight of about 62 kDa by SDS-PAGE under reducing conditions.
References
Yatohgo T, Izumi M, Kashiwagi H, et al. 1988, CELL STRUCTURE AND FUNCTION 13, 281-292.
Wojciechowski K, Chang CH, Hocking DC. Et al. 2004, Protein Expr. Purif. 36:131138.
Chen G, Gulbranson DR, Hou Z, et al. Nat Methods. 2011, 8(5): 424-429.
Recombinant human Vitronectin (VTN)
- Brand: StemImmune LLC
- Product CAT#: ETC-V-0010 (10ug), ETC-V-0100 (100ug), ETC-V-1000 (1000ug)
- Availability: In Stock
Available Options
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Tags: Media