Product Specifications

• Expression of Human Proteins in Human Cells
  
• Extreme low Endotoxin
• High Purity
• Animal Free and Xeno Free
• Tag Free

Source: Human cells derived
Structure: Glycosylated homotrimer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 17kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by the cytotoxicity in L-929 cell in the presence of actinomycin D.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Tumor necrosis factor alpha (TNF-α) is an EGF- related polypeptide growth factor that signals through the TNFRSF1A/TNFR1 and TNFRSF1B/ TNFBR. It is mainly secreted by macrophages and also produced by monocytes, keratinocytes, and various tumor cells.  TNF-α   stimulates the proliferation of a wide range of epidermal and epithelial cells. TNF-α is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia.  Under certain conditions it can stimulate cell proliferation and induce cell differentiation, impair regulatory T cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation.

References

Pennica D, et al. (1984) Nature 312, 724-729.
Nie H, et al. (2013) Nat. Med. 19, 322-328.
Friedmann E, et al. (2006) Nat. Cell Biol. 8,843- 848.