Product Application: Recombinant Human DC Cocktail
Primary Application:
- Dendritic Cell (DC) Generation: This cocktail is specifically formulated to induce the differentiation of monocytes into dendritic cells, which are key players in the immune system.
Specific Uses:
- Immunology Research: DCs are essential for studying immune responses, antigen presentation, and T cell activation.
- Vaccine Development: DCs can be used as a platform for developing vaccines, as they are highly effective at presenting antigens to the immune system.
- Cancer Immunotherapy: DCs can be engineered to present tumor antigens and stimulate anti-tumor immune responses.
- Autoimmune Disease Research: DCs play a role in autoimmune diseases, and this cocktail can be used to study their involvement in these conditions.
Key Features and Benefits:
- Optimized for DC Generation: The cocktail contains GM-CSF and IL-4 in optimized concentrations for efficient DC differentiation.
- High Purity and Low Endotoxin: Ensures optimal biological activity and minimal off-target effects.
- Animal-Free and Xeno-Free: Suitable for sensitive applications and avoids potential contamination.
- Tag-Free: Preserves the native structure and function of the cytokines.
- Convenient Formulation: Lyophilized format allows for easy storage and reconstitution.
In summary, the Recombinant Human DC Cocktail is a valuable tool for researchers and scientists working in the fields of immunology, vaccine development, cancer immunotherapy, and autoimmune disease research. It offers a convenient and efficient way to generate dendritic cells for various applications.
Welcome to the documentation download page. Below you'll find important documents related to our products, including Certificates of Analysis (COA), Data Sheets, Material Safety Data Sheets (MSDS), and more. These documents provide essential information for product use, safety, and technical specifications.
Product Specifications
- Expression of Human Proteins in Human Cells
- Extreme low Endotoxin
- High Purity
- Animal Free and Xeno Free
- Tag Free
Source: Human cells derived
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5 EU/ug
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein
Purity Confirmation: The protein was resolved by SDS-PAGE and the gel was stained with Coomassie blue.
Cocktail components
The cocktail contained recombinant human GM- CSF and IL-4. The concentrations of both cytokines had been optimized for dendritic cell culture.
Activity Assay
The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Reconstitution
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in 200ul sterile PBS or basic medium and add into appropriate volume of dendritic cell basic medium. The medium with DC cocktail supplement could be stored at 4°C for one month.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.
Recombinant Human DC cocktail
- Product CAT#: HCT-G4-0100 (100ml), HCT-G4-0500 (500ml)
- Availability: In Stock
Available Options
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