100bp DNA Ladder

REF: EG21903-S/M

Storage Condition

Store at 4℃ for 6 months, and at −20℃ for long-term storage.

Components

Component	EG21903S	EG21903M
100bp DNA Ladder	250 μl	5 × 250 μl

Description

The 100bp DNA Ladder contains 10 DNA fragments ranging from 100 bp to 1000 bp: 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 bp. Each 5 μl load provides ~50 ng DNA per band, except the 500 bp band which is ~120 ng for enhanced visibility.

Bands are designed with uniform intensity for clear resolution and approximate DNA quantification. Ladder is premixed with 1× loading buffer and tracking dyes, ready for direct electrophoresis.

Protocol

Load 5 μl of 100bp DNA Ladder per lane (adjust based on comb size).
Run in 1.2–2.0% agarose gel (REF: EG20910) with 1× TAE or 0.5× TBE buffer at 5–10 V/cm. Always use fresh gel and buffer.
Stain with Safe Red DNA Stain (REF: CP18106S) or other nucleic acid dyes; visualize under UV.
Ensure the bromophenol blue tracking dye runs to ~2/3 of the gel length.

Notice

Premixed with buffer and dyes; load directly without dilution.
Recommended gel concentration: ~1.5% TAE agarose; TBE can also be used for improved resolution.
Avoid repeated freeze–thaw cycles; aliquot before storage.