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DNA Assembly and Cloning

DNA Assembly & Cloning

Seamless/Gibson-style cloning with DNA Assembly Mix Ultra supports single- and multi-fragment assembly in as little as 5 minutes, using HiFi Taq DNA Ligase and an upgraded, high-stability formulation that tolerates heat, oxidation, freeze-thaw, and dry-ice exposure. A built-in transformation enhancer increases colony counts.

Highlights

  • Fast reactions: complete in ~5 min.
  • HiFi Taq DNA Ligase for higher-fidelity assembly.
  • Robust stability against heat/oxidation/freeze-thaw/dry-ice.
  • Supports single or multiple fragments (high positive rate, low mutation rate).
  • Transformation enhancer increases transformant yield.

Note: “TA/GC cloning” isn’t listed in the catalog; this page focuses on seamless assembly & ligases.

Product Compare (0)
2×Taq PCR Aurora Mix

2×Taq PCR Aurora Mix

REF: EG25104-M/L Storage Condition −20℃ Components Component..

All-in-One First-Strand Synthesis MasterMix (with dsDNase)

All-in-One First-Strand Synthesis MasterMix (with dsDNase)

All-in-One First-Strand Synthesis MasterMix (with dsDNase) REF: EG15133S Storage Condition ..

RTase III Primer Flexible All-in-One Mix (with dsDNase)

RTase III Primer Flexible All-in-One Mix (with dsDNase)

RTase III Primer Flexible All-in-One Mix (with dsDNase) REF: EG24102S Storage Condition -..

Taq-Plus PCR Forest Mix (2×) V2

Taq-Plus PCR Forest Mix (2×) V2

Taq-Plus PCR Forest Mix (2×) V2 REF: EG25105-M/L Storage Condition −20℃ ..

2× S705 HiFi Master Mix

2× S705 HiFi Master Mix

2× S705 HiFi Master Mix REF: EG24110-S/M/L Storage Condition −20℃ ..

2× S705 HiFi Master Mix (+Dye)

2× S705 HiFi Master Mix (+Dye)

2× S705 HiFi Master Mix (+Dye) REF: EG25103-S/M/L Storage Condition −20℃ ..

T4 DNA Ligase (Fast)

T4 DNA Ligase (Fast)

T4 DNA Ligase (Fast) REF: EG15205S Storage Condition −20℃ Components ..

DNA Assembly Mix Ultra

DNA Assembly Mix Ultra

DNA Assembly Mix Ultra REF: EG24204S Storage Condition −20℃ Components ..

Taq SYBR® Green qPCR Premix (Universal)

Taq SYBR® Green qPCR Premix (Universal)

Taq SYBR® Green qPCR Premix (Universal) REF: EG20117M Storage Condition Store at &m..

100bp DNA Ladder

100bp DNA Ladder

100bp DNA Ladder REF: EG21903-S/M Storage Condition Store at 4℃ for 6 months, and at &m..

1kb DNA Ladder

1kb DNA Ladder

1kb DNA Ladder REF: EG21909-S/M Storage Condition Store at 4℃ for 6 months. For long-te..

FY2000 DNA Marker

FY2000 DNA Marker

FY2000 DNA Marker REF: EG21910-S/M Storage Condition Store at 4℃ for 6 months. For long..

FY15000 DNA Marker

FY15000 DNA Marker

FY15000 DNA Marker REF: EG21912-S/M Storage Condition Store at 4℃ for 6 months. For lon..

FY5000 DNA Marker

FY5000 DNA Marker

FY5000 DNA Marker REF: EG21911-S/M Storage Condition Store at 4℃ for 6 months, and at &..

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