Product Specifications

• Expression of Human Proteins in Human Cells
• Extreme low Endotoxin
• High Purity
• Animal Free and Xeno Free
• Tag Free

Source: Human cells derived
Structure: Glycosylated monomer
Purity: >95% by SDS-PAGE
Endotoxin Level: <0.5EU/ug
Molecular Weight: 36kDa
Formulation: Lyophilized from a 0.2μm filtered solution in PBS without carrier protein

Activity Assay

The activity was measured by its ability to stimulate the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -80°C under sterile conditions after reconstitution.

Protein Description

Recombinant human erythropoietin (EPO) expressed in engineered human cells. EPO belongs to the EPO/TPO protein family. EPO is an acidic hormone mainly produced by kidneys. EPO promotes erythroid progenitor cell differentiation and initiates hemoglobin synthesis. EPO acts by binding to a specific erythropoietin receptor (EPOR) present on the red blood cell precursor in the bone marrow, stimulating these cells to transform into mature erythrocytes. The EPO sensitivity progressively increases with differentiation of immature progenitor cells.

References

Kitamura, T., et al. (1989) J. Cell Physiol. 140, 323-334.
Sinclair, A. M., et al. (2007) Cancer 110, 477-488.