DNA Amplification & PCR/qPCR
High-fidelity PCR for cloning and analysis, plus universal qPCR mixes that run on all major instruments. S705 Hi-Fi delivers approximately 70× Taq fidelity with fast cycling (as quick as ~5 s/kb), shows 3-day stability at 37 °C, and tolerates inhibitors and crude templates. Universal qPCR (SYBR) premixes include ROX by default—no instrument-specific tuning needed.
Highlights (PCR)
- ~70× Taq fidelity; ~5× Pfu speed with streamlined cycling.
- Extension rates up to ~5 s/kb on suitable targets.
- Robust stability after 3 days at 37 °C.
- Works across ~30–80% GC and with crude templates.
- (+Dye) versions allow direct gel loading of PCR products.
Highlights (qPCR)
- Universal SYBR qPCR mixes with ROX included—compatible with ABI, Bio-Rad, etc.
- Balanced chemistry for sensitivity and specificity.
- Supports high/low GC templates and routine gene expression assays.
Common Applications
- DNA amplification for cloning and sequence verification
- Genotyping and expression analysis (qPCR)
- Plasmid mapping, RFLP, cleanup, and sequencing library prep
Quick picker: choose a PCR mix
- S705 Hi-Fi → high fidelity + speed for cloning & long amplicons.
- PCR Master Mix (+Dye) → routine PCR with direct-load convenience.
Quick picker: choose a qPCR mix
- Universal qPCR (SYBR) → ROX-included for all instruments.
- ROX Dye (accessory) → for legacy setups that require separate ROX.
Tip: keep primer Tm within 58–62 °C and amplicons 70–200 bp for qPCR.
See also: Restriction Enzymes for vector prep and DNA Assembly & Cloning for downstream workflows.
2×Taq PCR Aurora Mix
REF: EG25104-M/L Storage Condition −20℃ Components Component..
All-in-One First-Strand Synthesis MasterMix (with dsDNase)
All-in-One First-Strand Synthesis MasterMix (with dsDNase) REF: EG15133S Storage Condition ..
RTase III Primer Flexible All-in-One Mix (with dsDNase)
RTase III Primer Flexible All-in-One Mix (with dsDNase) REF: EG24102S Storage Condition -..
Taq-Plus PCR Forest Mix (2×) V2
Taq-Plus PCR Forest Mix (2×) V2 REF: EG25105-M/L Storage Condition −20℃ ..
2× S705 HiFi Master Mix
2× S705 HiFi Master Mix REF: EG24110-S/M/L Storage Condition −20℃ ..
2× S705 HiFi Master Mix (+Dye)
2× S705 HiFi Master Mix (+Dye) REF: EG25103-S/M/L Storage Condition −20℃ ..
T4 DNA Ligase (Fast)
T4 DNA Ligase (Fast) REF: EG15205S Storage Condition −20℃ Components ..
DNA Assembly Mix Ultra
DNA Assembly Mix Ultra REF: EG24204S Storage Condition −20℃ Components ..
Taq SYBR® Green qPCR Premix (Universal)
Taq SYBR® Green qPCR Premix (Universal) REF: EG20117M Storage Condition Store at &m..