Primary Application:
Specific Uses:
Key Features and Benefits:
In summary, Human BMP-4 is a valuable tool for researchers and scientists working in the fields of stem cell biology, tissue engineering, regenerative medicine, and bone and cartilage research.
StemImmune recombinant human BMP-4 has been reported in 12 peer-reviewed studies involving chemical reprogramming, pluripotent stem-cell differentiation, mesoderm and hematopoietic induction, immune-cell development, neural stem-cell research, vascular biology, and primary human cell models. Available catalog numbers include HST-B4-0010, HST-B4-0100, and HST-B4-1000.
Liu Z, et al. Translational Psychiatry. Published April 1, 2026.
Research application: Neural stem-cell generation and evaluation in a preclinical neurodevelopmental research model. The publication reported StemImmune BMP-4 as a reagent used in the study’s stem-cell workflow.
Qiu J, et al. Autophagy. 2026. DOI: 10.1080/15548627.2026.2676795.
PubMed: 42153647
Research application: Vascular-injury and endothelial-biology research investigating the CD36-autophagy-lysosomal pathway. StemImmune BMP-4 was reported among the research reagents used in the study.
Peng F, et al. Cell Stem Cell. 2025;32(8):1192–1199.e11.
PubMed: 40744016
Research application: Chemical reprogramming of human blood cells into chemically induced pluripotent stem cells. The study used StemImmune BMP-4 during the reprogramming and pluripotent-cell-culture workflow.
Zhu S, et al. Experimental Hematology & Oncology. 2025;14(1):26.
PubMed: 40025520
Research application: Differentiation and functional enhancement of human pluripotent stem cell-derived eosinophils for solid-tumor immunotherapy research. StemImmune BMP-4 was used within the directed hematopoietic differentiation workflow.
Zhu J, et al. Cell Stem Cell. 2024;31(12):1732–1740.e6.
PubMed: 39442525
Research application: Chemically induced dedifferentiation and generation of expandable human limb-bud-like progenitor cells. StemImmune BMP-4 was reported as a component of the progenitor-cell generation workflow.
Wang S, et al. Cell. 2024;187:6152–6164.e18.
PubMed: 39326417
Research application: Generation and transplantation of chemically induced pluripotent stem-cell-derived pancreatic islets. StemImmune BMP-4 was used during the directed differentiation workflow.
Zhang Y, et al. Cell Reports Methods. 2024;4(9):100843.
PubMed: 39216483
Research application: Directed differentiation of human pluripotent stem cells into dual-attribute innate T/NK-like cells for cancer-immunotherapy research. StemImmune BMP-4 was used during early mesoderm and hematopoietic induction.
Liuyang S, et al. Cell Stem Cell. 2023;30(4):450–459.e9.
PubMed: 36944335
Research application: Rapid chemical reprogramming of human somatic cells into pluripotent stem cells. StemImmune BMP-4 was used as part of the chemical-reprogramming and pluripotent-cell establishment workflow.
Xu Y, et al. Cell Research. 2022;32(6):513–529.
PubMed: 35508506
Research application: Derivation and culture of totipotent-like stem cells capable of forming blastocyst-like structures. StemImmune BMP-4 was reported in the study’s stem-cell-culture and differentiation workflow.
Guan J, et al. Nature. 2022;605(7909):325–331.
PubMed: 35418683
Research application: Chemical conversion of human somatic cells into pluripotent stem cells. StemImmune BMP-4 was reported as a reagent used in the chemical-reprogramming and pluripotent-cell-culture process.
Lai W, et al. Stem Cell Reports. 2021;16(7):1697–1704.
PubMed: 34214485
Research application: Hematopoietic differentiation of human pluripotent stem cells into functional eosinophils for solid-tumor cytotoxicity research. StemImmune BMP-4 was used during the early differentiation workflow.
Xiang C, et al. Science. 2019;364(6438):399–402.
PubMed: 31023926
Research application: Long-term culture and functional maintenance of primary human hepatocytes in vitro. StemImmune BMP-4 was reported among the factors used in the hepatocyte-culture system.
Product use, concentration, catalog number, and protocol stage should be confirmed in each publication’s Methods, Key Resources Table, or Supplementary Information. StemImmune products are for research use only and are not intended for diagnostic or therapeutic use.
Access quality-control and technical documents for StemImmune recombinant human BMP-4, including Certificates of Analysis, the product data sheet, and the Safety Data Sheet.
Certificate of Analysis Search by product catalog number or lot number. 
Product Data Sheet View specifications, activity assay, reconstitution, storage, and application information. 
Safety Data Sheet View product handling, safety, storage, and disposal information. COA search: Use the product catalog number and applicable lot number printed on the vial label to locate the correct Certificate of Analysis.
StemImmune Recombinant Human BMP-4 is produced in human cells to support native protein folding, high biological activity, and reliable performance in demanding stem cell and developmental biology applications.
StemImmune Recombinant Human BMP-4 has been reported in peer-reviewed research published in journals including Nature, Cell, Science, Cell Stem Cell, and Cell Reports Methods. Reported applications include chemical reprogramming, stem-cell differentiation, mesoderm and hematopoietic induction, immune-cell development, neural stem-cell research, and hepatocyte culture.
Available catalog numbers: HST-B4-0010, HST-B4-0100, and HST-B4-1000.
View Published ResearchProduced in human cells to provide biologically relevant folding and strong performance in sensitive stem cell culture systems.
Widely used for iPSC/ESC differentiation, mesoderm induction, hematopoietic development, organoid culture, and regenerative medicine research.
Supplied as an animal-free, xeno-free, and tag-free recombinant protein to reduce unwanted variables in advanced cell culture workflows.
Tested for purity, endotoxin level, identity, and biological activity to support consistent experimental performance.
| Product Name | Recombinant Human BMP-4 |
|---|---|
| Expression System | Human Cells |
| Species | Human |
| Structure | Homodimer |
| Purity | >95% by SDS-PAGE |
| Endotoxin Level | <0.5 EU/μg |
| Molecular Weight | Approximately 34–40 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS without carrier protein |
| Animal Origin | Animal-Free / Xeno-Free |
| Tag | Tag-Free |
| Research Use | For Research Use Only. Not for diagnostic or therapeutic use. |
Every lot of StemImmune Recombinant Human BMP-4 undergoes quality control testing to support consistent performance in stem cell and developmental biology applications.
Purity Analysis:
Recombinant Human BMP-4 purity is determined by SDS-PAGE and is routinely greater than 95%.
Biological Activity:
Biological activity is measured by the ability of BMP-4 to induce alkaline phosphatase production in ATDC-5 mouse chondrogenic cells. In the representative assay shown above, the EC50 was approximately 5 ng/mL.
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
After reconstitution, prepare single-use aliquots and avoid repeated freeze-thaw cycles.
Bone Morphogenetic Protein 4 (BMP-4) is a member of the transforming growth factor beta family, which includes multiple structurally related bone growth factors. BMP-4 is widely expressed from early embryogenesis through adulthood and plays important roles in cartilage and bone formation, mesoderm induction, tooth development, limb formation, and fracture repair.
Mature human BMP-4 is a 116 amino acid protein and is normally found as a homodimer. Dimerization is facilitated by a disulfide bridge formed between the monomers, and each monomer contains three intrachain disulfide bridges arranged in a cystine knot motif.
BMP-4 signals through receptor complexes composed of type I and type II BMP receptors and downstream SMAD pathways. The bioavailability of BMP-4 is regulated by interactions with multiple proteins and glycosaminoglycans.
Activin A, FGF-basic / FGF2, VEGF165, TGF-β1, Noggin
SCF, FLT3 Ligand, TPO, IL-3, IL-6, VEGF165
BMP-4 is commonly used in stem cell differentiation, mesoderm induction, bone and cartilage research, organoid culture, and developmental biology studies.
StemImmune recommends reconstituting BMP-4 in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Yes. StemImmune Recombinant Human BMP-4 is produced as an animal-free and xeno-free recombinant protein.
No. This product is for research use only and is not intended for diagnostic or therapeutic use.
Zhang Y, et al. Blood. 2008;111:1933.
Shore EM, et al. Calcif. Tissue Int. 1998;63:221-229.
Nakamura K, et al. Exp. Cell Research. 1999;250:351-363.
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Product Specifications Expression of Human Proteins in Human Cells Extreme low Endotoxin H..
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Product Specifications Expression of Human Proteins in Human Cells Extreme low Endotoxin H..
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Product Specifications Expression of Human Proteins in Human Cells Extreme low Endotoxin H..
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Product Specifications Expression of Human Proteins in Human Cells Extreme low Endotoxin H..
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Product Specifications Expression of Human Proteins in Human Cells Extreme low Endotoxin H..
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Source: E.coli derived Structure: Monomer Purity: >95% by SDS-PAGE Endotoxin Level: <1EU/ug..
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Product Specifications Expression of Human Proteins in Human Cells Extreme low Endotoxin H..
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Tags: Growth Factors