Primary Application:
Specific Uses:
Key Features and Benefits:
In summary, Human Activin A is a versatile tool for researchers and scientists working in the fields of stem cell biology, tissue regeneration, inflammation, neural development, and reproductive biology.
StemImmune recombinant human Activin A has been reported in seven peer-reviewed studies involving chemical reprogramming, pluripotent stem-cell culture, pancreatic islet differentiation, hematopoietic differentiation, epigenetic regulation, and primary human cell models.
Wang Y, et al. Cell Stem Cell. 2025;32(8):1192–1199.e11.
PubMed: 40744016
Research application: Chemical reprogramming of human blood cells into chemically induced pluripotent stem cells. The study used StemImmune Activin A during the reprogramming and pluripotent-cell culture workflow.
Wang S, et al. Cell. 2024;187(22):6152–6164.e18.
PubMed: 39326417
Research application: Generation and transplantation of chemically induced pluripotent stem-cell-derived pancreatic islets for type 1 diabetes research. Activin A was used during the directed differentiation workflow.
Guan J, et al. Nature. 2022;605(7909):325–331.
PubMed: 35418683
Research application: Small-molecule conversion of human somatic cells into pluripotent stem cells without introducing exogenous transcription factors. StemImmune Activin A supported the chemical reprogramming and culture process.
Gao X, et al. Nature Communications. 2021;12:3017.
PubMed: 34021145
Research application: Development of chemically defined and xeno-free culture conditions for human extended pluripotent stem cells. The study reported StemImmune Activin A, Catalog No. HST-A-1000.
Zhu Y, et al. Stem Cell Reports. 2021;16(7):1697–1704.
PubMed: 34214485
Research application: Directed differentiation of human pluripotent stem cells into eosinophils and evaluation of their antitumor activity against solid tumors. Activin A was included in the early differentiation workflow.
Yan P, et al. Cell Reports. 2020;32(1):107870.
PubMed: 32640235
Research application: Genome-wide analysis of R-loop changes during cellular differentiation and reprogramming. Activin A was used in the stem-cell differentiation and cell-state transition workflow.
Zhang K, et al. Science. 2019;364(6438):399–402.
PubMed: 31023926
Research application: Long-term culture and functional maintenance of primary human hepatocytes in vitro. Activin A was used in the hepatocyte culture and cell-state maintenance system.
Publication information is provided for research reference. Please review the original articles and supplementary methods for complete experimental conditions, concentrations, and catalog-number details.
Access quality-control and technical documents for StemImmune recombinant human Activin A, including Certificates of Analysis, the product data sheet, and the Safety Data Sheet.
COA search: Use the product catalog number and applicable lot number printed on the vial label to locate the correct Certificate of Analysis.
StemImmune Recombinant Human Activin A is produced in human cells to support biologically relevant protein folding, high biological activity, and reliable performance in stem cell differentiation and developmental biology workflows.
Produced in human cells to support biologically relevant folding and activity for advanced stem cell and cell culture workflows.
Activin A is widely used for definitive endoderm induction, mesendoderm specification, human iPSC and ESC differentiation, and organoid culture.
Designed to reduce unwanted variables in sensitive stem cell, developmental biology, and regenerative medicine research applications.
Tested for purity, endotoxin level, identity, and biological activity. Typical EC50: 6.68–10.02 ng/mL in an MPC-11 cell proliferation inhibition assay.
| Product Name | Recombinant Human Activin A Protein |
|---|---|
| Catalog Numbers | HST-A-0010 — 10 μg HST-A-0100 — 100 μg HST-A-1000 — 1 mg |
| Gene Symbol | INHBA |
| UniProt ID | P08476 |
| Protein Family | TGF-β Superfamily |
| Species | Human |
| Expression System | Human cells |
| Protein Structure | Disulfide-linked homodimer |
| Molecular Weight | Approximately 25 kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin Level | <0.5 EU/μg |
| Biological Activity | Determined by inhibition of murine MPC-11 cell proliferation |
| Typical EC50 | 6.68–10.02 ng/mL |
| Formulation | Lyophilized from a sterile-filtered solution in PBS without carrier protein |
| Carrier Protein | None |
| Animal Origin | Animal-Free / Xeno-Free |
| Tag | Tag-Free |
| Recommended Reconstitution | Sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin |
| Storage | Store lyophilized protein at -20°C to -80°C. Avoid repeated freeze-thaw cycles after reconstitution. |
| Available Sizes | 10 μg, 100 μg, and 1 mg |
| Product Grade | Research Use Only |
The biological activity of StemImmune Recombinant Human Activin A is determined by its ability to inhibit the proliferation of the murine MPC-11 plasmacytoma cell line.
Each production lot is tested using an MPC-11 cell proliferation inhibition assay to confirm functional Activin A activity.
Purity is greater than 95% as determined by SDS-PAGE.
Endotoxin level is less than 0.5 EU/μg.
Production lots are evaluated for purity, endotoxin level, identity, and biological activity before release.
Briefly centrifuge the vial before opening. Reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin .
After reconstitution, prepare single-use aliquots and avoid repeated freeze-thaw cycles.
Activin A is a member of the TGF-β superfamily and is involved in biological processes including embryonic development, tissue morphogenesis, tissue repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive biology, and cancer research.
In stem cell research, Activin A is widely used to regulate pluripotent stem cell differentiation and to support definitive endoderm and mesendoderm induction. It is commonly combined with growth factors such as BMP-4, FGF2, and Wnt pathway modulators in directed differentiation protocols.
Activin A is commonly used for stem cell differentiation, definitive endoderm induction, mesendoderm specification, organoid culture, developmental biology, and regenerative medicine research.
Yes. StemImmune Recombinant Human Activin A is produced in human cells.
Biological activity is determined using an MPC-11 cell proliferation inhibition assay. The typical EC50 is 6.68–10.02 ng/mL under the stated assay conditions.
StemImmune recommends reconstituting Activin A in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
No. This product is for research use only and is not intended for diagnostic or therapeutic use.
Kumanov P, et al. Reprod. Biomed. Online. 2005;10:786.
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